何首乌饮对大鼠睾丸间质细胞类固醇激素合成急性调节蛋白和细胞色素P450胆固醇侧链裂解酶蛋白表达的影响  

Effect of Heshouwuyin on protein expression of steroidogenic acute regulatory protein and cytochrome P450 cholesterol side chain cleavage enzyme in Leydig cells of rats

作　　者：王建明[1,2] 孙静[1] 齐峰[3] 宋庆亮[1] 牛嗣云[1] 回陈红 徐天戎 贺飞[1] 

机构地区：[1]河北大学医学院组织学胚胎学教研室,河北保定071000 [2]河北民族师范学院人事处,河北承德067000 [3]保定市第一中医院呼吸科,河北保定071000

出　　处：《解剖学报》2017年第1期30-36,共7页Acta Anatomica Sinica

基　　金：国家自然科学基金(81373187);河北省自然科学基金(H2013201139)

摘　　要：目的探究何首乌饮影响大鼠睾丸间质(Leydig)细胞类固醇激素合成急性调节蛋白(St AR)和细胞色素P450胆固醇侧链裂解酶(P450scc)蛋白表达的变化。方法选用10~20 d的幼年雄性Wistar大鼠,分离、培养Leydig细胞,然后收集。采用氧化损伤的方法建立Leydig细胞衰老模型。实验分组:间质细胞衰老模型组,何首乌饮处理组,首乌丸处理组,正常组和何首乌饮对照组。结果衰老模型组Leydig细胞St AR和P450scc的免疫组织化学染色强度明显低于正常组和何首乌饮对照组(P<0.01)。另一方面,何首乌饮和何首乌丸干预组St AR和P450scc的免疫组织化学染色强度明显高于衰老模型组(P<0.05),何首乌饮组好于何首乌丸组。结论何首乌饮可提高睾丸Leydig细胞St AR和P450scc蛋白的表达,提高睾酮的合成能力,从而延缓Leydig细胞衰老。Objective To explore the effect of Heshouwuyin on the steroidogenic acute regulatory protein（St AR）and cytochrome P450 cholesterol side chain cleavage enzyme（P450scc） protein expression in Leydig cells of rats.Methods Juvenile male Wistar rat,10-20 days old,were used to isolate the Leydig cells and cultured,and then collected them.The oxidative damage technique was used to establish Leydig cell aging model.This study had a Leydig aging model,Heshouwuyin and Shouwu pill treatments,and normal and Heshouwuyin control group.Results The intensities of immunohistichemical staining for St AR and P450 scc were significantly lower in the Leydig aging model group than those in the normal group and Heshouwuyin control group（P 〈 0.01）,and were significantly higher in the Heshouwuyin and Shouwu pill group than that in the aging model group（P 〈 0.05）.Heshouwuyin group was better than Shouwu pill group.Conclusion Heshouwuyin can increase the protein expression of St AR and P450 scc in Leydig cells,improve the synthesis of testosterone,thus delaying the senescence of Leydig cells.

关 键 词：何首乌饮 睾丸Leydig细胞 免疫组织化学 免疫印迹法 类固醇激素合成急性调节蛋白 P450胆固醇侧链裂解酶 大鼠 

分 类 号：R384.1[医药卫生—医学寄生虫学]