白术醇提物对佐剂性关节炎大鼠足跖肿胀度和炎性细胞因子的影响  

The Effect of Ethanol Extract of Atractylodes Macrocephala Koidz. on Paw Edoma and Iinflammaroty Cytokines in Rats with Adjuvant Arthritis

作　　者：赵桂芝[1,2] 徐攀[1,2] 浦锦宝[1,2] 梁卫青[1,2] 胡轶娟[1,2] 魏克民[1,2] ZHAO Guizhi XU Pan PU Jinbao et al(Zhejiang Academy of Traditional Chinese Medicine, Hangzhou（310007 Key Laboratory of Research and Devel- opment of Chinese Medicine of Zhejiang Province)

机构地区：[1]浙江省中医药研究院,杭州310007 [2]浙江省中药新药研发重点实验室

出　　处：《浙江中医药大学学报》2017年第1期32-37,共6页Journal of Zhejiang Chinese Medical University

基　　金：浙江省中药新药研发重点实验室(2008F3036);浙江省中医药科技计划(2013ZA006)~~

摘　　要：[目的]研究白术醇提物对佐剂性关节炎(adjuvant arthritis,AA)大鼠足跖肿胀度和炎性细胞因子的影响,并探讨可能的作用机制。[方法]将60只SD雌性大鼠随机分成6组,即空白组、模型组、阳性药组(阿司匹林)、白术醇提物低、中、高剂量组(0.25、0.5、1g/(kg·d))。除空白组外,其余各组用弗氏完全佐剂诱导建立大鼠AA模型,测量造模前后不同时间点的足跖肿胀度,ELISA法检测大鼠血清和炎性组织中的TNF-α、IL-1β和PGE2含量。[结果]与空白组相比,模型组大鼠的足跖肿胀度、血清和炎性组织中的炎症因子TNF-α、IL-1β和PGE2含量显著增加。与模型组相比,白术醇提物能剂量依赖性抑制AA大鼠的原发性足跖肿胀度,对继发性的足跖肿胀也有一定程度的抑制,能明显抑制AA大鼠血清和炎性组织中的TNF-α、IL-1β和PGE2的含量。[结论]白术醇提物对于佐剂性关节炎大鼠具有较好的保护作用,并且随着剂量的增大,保护作用增强,抑制炎症细胞因子TNF-α、IL-1β和PGE2合成和释放可能是其主要的作用机制之一。[Objective] To investigate the impact on paw edema and inflammaroty cytokines of the adjuvant arthritis rats by ethanol extract of Atractylodes macrocephala Koidz.（EAM）, and explore the possible mechanism. [Methods] SD rats induced by Freunds complete adjuvant were randomly divided into 6groups： normal group, model group, positive drug group and three EAM treated groups（respectively with high-, mid- and low-dosages）. Paw edema was measured by thickness gauge, and TNF-α, IL-1β and PGE2 of serum and swelling paw were detected by ELISA methods. [Result] Compared with normal group, the primary and secondary paw edema of adjuvant arthritis rats showed extremely significant differences, and the level of TNF- α, IL-1β and PGE2 in the serum and swelling paw increased obviously. Compared with model group, EAM with different dosages could respectively decrease the primary and secondary paw edema degree and the level of inflammatory cytokines. [Conclusion] EAM had certain protection effects on adjuvant arthritis, which became stronger with the dosage of ethanol extracts going up. The inhibition of TNF- α, IL-1β and PGE2 may be an important mechanism for these effects.

关 键 词：白术 醇提物 AA大鼠 足跖肿胀度 炎性细胞因子 剂量依赖 

分 类 号：R331[医药卫生—人体生理学]