UPLC法检测HNHHJ-1菌株发酵液中2-羟基联苯含量  

Determination of 2-Hydroxybiphenyl Content in Fermentation Broth of Desulfurization Strain HNHHJ-1 by UPLC Method

作　　者：张宇[1] 张玥[1] 马宁[1] 王志成[1] 

机构地区：[1]黑龙江省科学院能源环境研究院,黑龙江哈尔滨150090

出　　处：《化学与粘合》2017年第1期40-42,共3页Chemistry and Adhesion

基　　金：黑龙江省青年科学基金(编号:QC2014C028)

摘　　要：采用超高效液相色谱法(UPLC)测定脱硫菌发酵液中2-羟基联苯(2-HBP)的含量。色谱柱为ACQVITY DPLC@BEH C18(2.1×50mm,1.7μm,Waters),流动相为甲醇∶水(90∶10,V/V),柱温30℃,流速0.2m L/min,进样量2μL,PDA检测器检测2-HBP,检测器检出限范围为0~200mg/L。结果显示2-HBP的保留时间为0.74min。利用该检测方法得到HNHHJ-1菌株培养后的发酵液中2-HBP的含量为5.35mg/L。The concentration of 2-hydroxybiphenyl （2-HBP） in the fermentation broth of desulfurization strain was determined by ultra performance liquid chromatography （UPLC）. The 2-HBP was separated by a chromatographic column （2.1 ×50 ram, 1.71μm, waters）, eluted with methyl alcohol and water with a volume ratio of 90：10 at a flow rate of 0.2mL/min and detected with PDA detector; the column temperature was 30℃, and the sample quantity was 2μL. The results showed that the retention time of 2-HBP was 0.74min with a detection limit of 0-200mg/L. The 2-HBP concen- tration in the fermentation broth of strain HNHHJ-1 was 5.35mg/L.

关 键 词：超高效液相色谱法 2-HBP 检测 

分 类 号：O657.72[理学—分析化学]