双向电泳技术筛选鉴定冬虫夏草中指标性蛋白质研究  

Study on Characteristic Proteins and Identification of Ophiocordyceps sinensis Using 2-D Electrophoresis

作　　者：童芯锌[1] 王艺璇[1] 陈璐[1] 邱乙 万德光[1] 任艳[1] 彭成[1] 沈才洪 李军德[3] 国锦琳[1,2] TONG Xinxin WANG Yixuan CHEN Lu QIU Yi WAN Deguang REN Yan PENG Cheng SHEN Caihong LI Junde GUO Jinlin(Key Laboratory of Standardization of Chinese Medicine, Ministry of Education, Chengdu University of Traditional Chinese Medicine, Chengdu , 611137, China LuzhouLaoJiao Co., Ltd., Luzhou, 646000, China National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing , 100700, China)

机构地区：[1]成都中医药大学中药材标准化教育部重点实验室,四川成都611137 [2]泸州老窖博士后工作站,四川泸州646000 [3]中国中医科学院中药资源中心,北京100700

出　　处：《中国现代中药》2017年第1期26-30,36,共6页Modern Chinese Medicine

基　　金：国家自然科学基金(30801522;81373920)

摘　　要：对名贵中药材冬虫夏草及其伪品建立稳定可靠的双向电泳鉴定方法。正品冬虫夏草中共同表达的蛋白质斑点为88个,伪品稳定差异表达的蛋白质斑点为21个,提示可作为下一步冬虫夏草鉴定的指标性组分。进一步质谱分析与NCBI数据库比对表明:6种蛋白仅来自于菌数据库,9种蛋白仅来自于昆虫数据库,12种蛋白仅来自于全库,3种蛋白昆虫和菌的数据库均能检索,1种蛋白仅来自于菌数据库和全库,提示下一步可进行冬虫夏草指标性蛋白质功能研究,为冬虫夏草有效成分研究奠定基础。The study is aimed to establish an efficient two-dimensional gel electrophoresis protocol for proteomic studying of Ophiocordyceps sinensis. Using two-dimensional electrophoresis, a proteomic analysis of O. sinensis from different areas and other Cordyceps fungi were compared. 88 kinds of proteins in O. sinensis were found. Compared with other Cordyceps fungi, 21 kinds of characteristic proteins spots in O. sinensis were found. These indicated that the 21 kinds of proteins were able to be used to authenticate O. sinensis from other Cordyceps fungi. Then further analysis of the proteins Mass Spectra Database of NCBI, 6 kinds of proteins came from the fungi database, 6 kinds of proteins from insect database, 12 kinds of proteins from the total database, 3 kinds of proteins were found both in insect database and fungi database, 1 kinds of proteins was found in the fungi database and total database. These indicate that further study is needed for some function protein.

关 键 词：冬虫夏草 双向电泳 指标性蛋白质 功能蛋白质 

分 类 号：R817[医药卫生—影像医学与核医学]