鹿茸:干细胞与其微环境相互的产物  

Deer Antler: Derivative of Interactions between Antler Stem Cells and Niche

作　　者：杨言慧[1,2] 焦晶雪[2] 李军德[3] 李春义[1] YANG Yanhui JIAO Jingxue LI Junde LI Chunyi(Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, State Key Lab for Molecular Biology of Special Animals, Changchun 130112, China The 2nd Clinic Hospital of Jilin University, Changchun 130041, China State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijng 100700, China)

机构地区：[1]中国农业科学院特产研究所,特种动物分子生物学省部共建国家重点实验室,吉林长春130112 [2]吉林大学第二医院,吉林长春130041 [3]道地药材国家重点实验室培育基地,中国中医科学院,中药资源中心,北京100700

出　　处：《中国现代中药》2017年第1期31-36,共6页Modern Chinese Medicine

基　　金：国家高技术研究发展计划(863)项目(2011AA100603);国家自然科学基金项目(31170950);吉林省重点科技攻关项目(20150204071NY);吉省自然科学基金项目(20140101139JC)

摘　　要：鹿茸的周期性再生是一个基于干细胞的过程,鹿茸干细胞(ASC)存在于生茸区骨膜(AP)与角柄骨膜(PP)中。本篇综述首先提出了一个假说,即紧密包裹的皮肤是ASC微环境的主要组成成分,之后通过一系列实验研究对这一假说进行了验证。通过插膜实验证明ASC和其紧密包裹皮肤之间的相互作用是鹿茸发生与再生不可或缺的,这一相互作用是通过交换游离小分子物质而实现的。AP组织的皮内移植实验表明,表皮和真皮毛乳头细胞参与了这一相互作用的过程。进一步的AP组织翻转实验表明,尽管自然情况下AP的纤维层紧贴皮肤,但诱发鹿茸发生和再生的信号来源于AP组织细胞层的细胞。通过实验改变鹿茸干细胞的微环境对鹿茸的发生产生了显著的影响。我们相信最终对这些相互作用分子的鉴别和分离不仅能够使我们进一步揭示鹿茸发生和再生的机制,更对再生医学有着重要意义。Full regeneration of deer antlers is a stem cell-based process, and antler stem cells （ASC） reside in both the antlerogenic periosteum（AP） and pedicle periosteum （PP）. In this review, we first advanced a hypothesis that the clesely associated skin is the primary component of ASC niche and then provided results for testing of this hypothesis. Membrane insertion experiments demonstrated that interactions between the ASC and the associated skin are indispensible for both antler generation and regeneration, and these are realized through exchanging diffusible molecules. Intradermal AP graft study showed that both epidermal ce｜｜s and dermal papilla cells of deer skin are involved in these interactions. Further, the AP inversion experiment indicated that the initial inductive signal originates from the ASC resident in the AP cellular layer, although the AP fibrous layer is naturally adjacent to skin. Experimental manipulation to the ASC niche has profound effects on antler development. We believe that eventual identification of these interactive molecules would not only greatly enhance our knowledge of antler development, but also have significant impacts on regenerative medicine in general.

关 键 词：鹿茸 角柄 鹿 干细胞 骨膜 组织相互作用 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]