三种鸡菌属真菌核苷及嘌呤碱基类成分含量测定  

Determination of Nucleoside and Purine Bases in Three Kinds of Fruiting Body of Termitomyces

作　　者：高艳云[1,2] 张园娇 张倩[2] 陈建伟[2] GAO Yanyun Zhang Yuanjiao Zhang Qian CHEN Jianwe(Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029 School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023)

机构地区：[1]南京中医药大学附属医院,江苏南京210029 [2]南京中医药大学药学院,江苏南京210023

出　　处：《中国现代中药》2017年第1期68-72,共5页Modern Chinese Medicine

基　　金：江苏高校优势学科建设工程资助项目(PAPD);我国水生;耐盐中药资源的合理利用研究行业专项(201407002)

摘　　要：目的:以高效液相色谱法测定鸡、灰鸡和黄鸡3种鸡菌子实体中核苷及嘌呤碱基类成分的组成和含量,建立鸡类真菌核苷及嘌呤碱基类成分的控制指标。方法:采用Waters Xbridge C_(18)柱(250 mm×4.6 mm,5μm),以甲醇-0.1%磷酸水溶液为流动相,流速为0.8 mL·min^(-1),柱温为30℃,检测波长为260 nm。结果:3种鸡菌子实体中均检出次黄嘌呤、黄嘌呤、胞苷、腺苷、鸟苷、尿苷、肌苷等成分,且在质量浓度分别在7.51~120.12、4.99~79.84、25.13~402.00、12.61~201.80、5.03~80.48、5.00~80.00、10.00~160.00μg·mL^(-1)与峰面积呈良好线性关系(r≥0.999 6);平均回收率为95.83%~103.01%,RSD≤3.98%(n=6)。鸡中2种嘌呤碱基、5种核苷类成分含量分别为:0.36、3.13、2.20、3.63、1.11、1.87、1.50 mg·g^(-1);灰鸡含量为:0.10、0.78、1.86、3.07、1.48、1.70、0.62 mg·g^(-1);黄鸡含量为:0.22、2.53、1.86、2.35、0.78、0.84、0.83 mg·g^(-1)。结论:本实验建立的HPLC测定鸡类真菌中5种核苷及2种嘌呤碱基类成分含量的分析方法简单、重复性好、准确度高,可以为鸡类真菌的质量控制提供参考,3种鸡菌子实体在医疗和营养保健上都有较高的研究价值。Objective： To establish an HPLC method for the determination of nucleoside components in the fruiting body of Termitomyces albuminosus, T. eurrhizus and T. aurantiacus. Methods： The assay was performed on a Waters Xbridge Cls （250 mm × 4. 6 mm, 5 μm） column, mobile phase consisted of methanol - 0. 1% orthophosphoric acid water with gradient elution at the flow rate of 0. 8 mL·min-1, column temperature was 30 ℃ , and the detection wavelength was set at 260 nm. Results： The three kinds of fruiting body of Termitomyces all checked out 2 kinds of purine base and 5 kinds of nucleoside, including hypoxanthine, xanthine, cytidine, adenosine, guanosine, uridine, inosine, and they showed good linearity （ r≥ 0. 999 6） in the range of 7.51-120. 12, 4. 99-79.84, 25.13-402.00, 12. 61-201.80, 5.03-80. 48, 5.00-80. 00, 10. 00- 160. 00 μg· mL-1, respectively. The average recoveries （ n = 6 ） were within 95.83% -103.01% with RSD ≤ 3.98% . The contents of 2 kinds of purine base and 5 kinds of nucleoside components in the fruiting body of T. albuminosus were 0. 36, 3.13, 2. 20, 3.63, 1.11, 1.87, 1.50 mg·g-1 respectively, they in the fruiting body of T. eurrhizus were 0. 10 0.78 1.86, 3.07, 1.48, 1.70, 0. 62 mg· g-1 respectively, and they in the fruiting body of T. aurantiacus were 0. 22, 2. 53, 1.86, 2. 35, 0. 78, 0. 84, 0. 83 mg·g-1, respectively. Conclusion： This HPLC method was simple, accurate and reducible, and can be used for quality evaluation and quality control of Termitomyces. The three kinds of fruiting body of Termitomyces would have higher study value for medicine, nutrition and heahh food.

关 键 词：鸡纵菌属 核苷类 嘌呤碱基 HPLC 含量测定 

分 类 号：R284.1[医药卫生—中药学]