芪桂益脉灵对大鼠缺血再灌注损伤心肌组织PI-3Kp85a mRNA、AKT2 mRNA表达的影响  

Effects of Qigui Yimailing(芪桂益脉灵)on PI-3Kp85a mRNA and AKT2 mRNA Expressions in Myocardial Cells of Rats with Acute Ischemia-Reperfusion Injury

作　　者：张超越[1] 赵龙[1] 徐京育[2] 李书霖 ZHANG Chaoyue ZHAO Long XU Jingyu LI Shulin(Heiloniang University of Chinese Medicine ,Harbin,Heilong]iang 150040 First Hospital Affiliated to Heilongjiang University of Chinese Medicine ,Harbin ,Heilongjiang 150040 HeiLongJiang Provincial Administration of Chinese Medicine ,Harbin ,Heilongjiang 150036)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040 [3]黑龙江省中医药管理局,黑龙江哈尔滨150040

出　　处：《中国中医药科技》2017年第1期27-29,共3页Chinese Journal of Traditional Medical Science and Technology

基　　金：黑龙江省自然科学基金项目(H201326)

摘　　要：目的:探讨芪桂益脉灵(QGYML)对急性心肌缺血再灌注损伤大鼠心肌组织PI-3Kp85a(磷酸肌醇-3激酶)、AKT2(丝氨酸-苏氨酸激酶)mRNA的影响。方法:取SD大鼠50只,随机分为5组,正常组、假手术组、模型组、芪桂益脉灵低剂量组和芪桂益脉灵高剂量组,分别给予生理盐水、QGYML 0.6、1.2 g/kg灌胃,每天1次,连续灌胃7 d。处死大鼠,取心尖部缺血组织,采用半定量逆转录-聚合酶链反应(RT-PCR)方法测定PI-3Kp85a mRNA、AKT2 mRNA的表达。结果:与正常组、假手术组比较,模型组大鼠心肌组织PI-3Kp85a mRNA、AKT2 mRNA表达明显减弱,芪桂益脉灵组PI-3Kp85a mRNA、AKT2m RNA表达明显增强,与模型组比较具有统计学意义(P<0.05),高剂量组的效果更加显著。结论:芪桂益脉灵可显著提高大鼠缺血再灌注损伤心肌组织PI-3Kp85a mRNA、AKT2 mRNA的表达水平,对心肌具有保护作用。Objective： To explore the effects of Qigui Yimailing（QGYML） on PI -3Kp85a mRNA and AKT2 mRNA expressions in myocardial tissue of rat with ischemia reperfusion injury. Methods：50 SD male rats were randomly divided into 5 groups ,each had 10 rats. The normal group（N） after 7 days physiological saline ir- rigation didn＇t do any processing, the sham operation group （SH） after 7 days physiological saline irrigation took the thoracotomy to expose left coronary artery of the rats ,thread but did not make ligation, the rest rats were di- vided into pure ischemia reperfusion group , QGYML low dose group（QL） and QGYML high dose group（ QH）, with the physiological saline,QGYML（ 1.2 g/kg and 0. 6 g/kg） respectively,whose irrigation were served 7 days in a row during the morning. Acute myocardial infarction（AMI） models were caused by ligation of the left anteri- or descending branch of the coronary artery in the successful rat models＇ hearts. After execution of rats, RT - PCR was used to determine the expressions of PI -3Kp85a mRNA and AKT2 mRNA in the tip of the rat cardiac is- chemic tissue. Results：PI -3Kp85a mRNA and AKT2 mRNA expressions of model group were significantly re- duced, the differences were statistic significant to compare with normal or sham group（ P 〈 0. 05 ） ;PI -3Kp85a mRNA and AKT2 mRNA expressions of QGYML groups were obviously higher than those of model group （P 〈 0. 05 ）, and the effect of QGYML was more significant. Conclusion： QGYML can improve the expressions of PI - 3Kp85a mRNA and AKT2 mRNA in the myocardial tissue of rats with ischemia -reperfusion injury, which can protect damaged myocardium.

关 键 词：缺血再灌注 心肌损伤 磷酸肌醇-3激酶 丝氨酸-苏氨酸激酶 芪桂益脉灵 大鼠 

分 类 号：R285.5[医药卫生—中药学]