脐带间充质干细胞在2型糖尿病大鼠中的疗效及作用机制  

Effect of human umbilical cord-derived mesenchymal stem cells on type 2 diabetic rats and its mechanism

作　　者：高杰清[1] 谢宗燕[1] 母义明[1] 郝好杰[1] 谢敏[1] 程愈[1] 张琪[1] 

机构地区：[1]解放军总医院内分泌科,北京100853

出　　处：《中华内分泌代谢杂志》2017年第1期62-67,共6页Chinese Journal of Endocrinology and Metabolism

基　　金：国家高技术研究发展计划（863计划,2013AA020105）

摘　　要：目的探讨脐带间充质干细胞（hMSC）在2型糖尿病大鼠中的疗效及作用机制。方法高脂饲养和小剂量链脲佐菌素诱导建立2型糖尿病模型，经尾静脉输注hMSC或等量10倍浓缩的hMSC培养上清后，每日检测实验大鼠血糖。治疗后第7天行腹腔注射葡萄糖耐量试验和胰岛素耐量试验。胰腺组织行胰岛素/胰升糖素免疫荧光染色。结果干细胞注射后，糖尿病大鼠的空腹血糖和稳态模型评估的胰岛素抵抗指数（HOMA-IR）下降（均P〈0.01），糖耐量及胰岛素敏感性均明显改善（均P〈0.01），胰岛内β细胞数量增加。尾静脉输注10倍浓缩hMSC培养上清可在糖尿病大鼠中发挥类似hMSC的治疗效应。结论在2型糖尿病大鼠中尾静脉输注hMSC能有效降低血糖、改善胰岛素敏感性，并增加胰岛内β细胞数量，该疗效可能源于hMSC的分泌效应。Objective To observe the effect of human umbilical cord-derived mesenchymal stem cells（hMSCs）on type 2 diabetic rats, and to explore the possible mechanism.Methods Type 2 diabetic rats were induced by high-fat diet combined with a low dosage of streptozotocin（STZ, 25 mg/kg）. After 3×106hMSCs suspended in 1 ml PBS or 1ml 10-fold concentrated hMSC supernatant were intravenously infused into the rats via the tail vein, the blood glucose levels were measured every day. One week later, intraperitoneal glucose tolerance test and insulin tolerance test were performed to evaluate the effects of hMSCs on diabetic rats. Pancreatic tissues were collected for insulin/glucagon immunofluorescence staining. Results After hMSCs infusion, blood glucose level and homeostasis model of assessment for insulin resistance index were significantly decreased in type 2 diabetic rats（both P〈0.01）. The glucose tolerance and insulin tolerance were greatly alleviated by hMSCs（all P〈0.01）. Intravenously infused 1ml 10-fold concentrated hMSC supernatant showed a similar result to hMSCs.Conclusion In type 2 diabetic rats, hMSCs are able to effectively lower the blood glucose level, improve insulin sensitivity, and increase the number of β cells, which seems to be mediated by their secreted molecules.

关 键 词：间充质干细胞 糖尿病 胰岛素抵抗 胰岛素分泌细胞 

分 类 号：R587.1[医药卫生—内分泌]