陆地棉光敏雄性不育基因ys-1的遗传分析与初步定位  

Genetic Analysis and Preliminary Mapping of the Photoperiod-Sensitive Male Sterility Gene ys-1 in Upland Cotton

作　　者：张蒙[1] 刘记[1] 魏恒玲[1] 庞朝友[1] 王寒涛[1] 范术丽[1] 王晖[1] 宋美珍[1] 喻树迅[1] Zhang Meng Liu Ji Wei Hengling Pang Chaoyou Wang Hantao Fan Shuli Wang Hui Song Meizhen Yu Shuxun(Institute of Cotton Research of Chinese Academy of Agricultural Sciences / State Key Laboratory of Cotton Biology, Anyang, Henan 455000, Chin)

机构地区：[1]中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南安阳455000

出　　处：《棉花学报》2017年第1期9-16,共8页Cotton Science

基　　金：国家自然科学基金(31501344)

摘　　要：【目的】精细定位和克隆陆地棉光敏雄性不育基因。【方法】从陆地棉中040029的航天诱变后代中选育出新型光敏雄性不育系中9106,以中9106与11个陆地棉材料配制杂交组合,初步分析了中9106的杂种优势。以中9106为母本与陆地棉乐土603构建遗传分离群体F1、F2,分析不育性状的遗传特征。利用集团分离分析法筛选简单序列重复(Simple sequence repeat,SSR),并在包含186个单株的中9106×乐土603 F2群体中用上述SSR初步定位不育基因。【结果】中9106为母本×陆地棉乐土603的F1单株育性均表现正常,而F2群体中的可育单株数∶不育单株数符合3∶1的分离比,推测中9106的不育性状受1对隐性核基因控制。利用集团分离分析法从16 544对SSR中筛选到18对与该不育基因连锁的标记。利用中9106×乐土603的F2群体和18对SSR标记,将不育基因定位于D12染色体,位于标记NAU3442与CGR6339之间,遗传距离均为0.2c M,将该不育基因命名为ys-1。【结论】本研究有助于ys-1的精细定位及其克隆。[Objective] The aim of this study was to finely map and clone the photoperiod-sensitive male sterility gene in upland cotton. [Method] Eleven male parents were crossed with CCRI 9106 （i.e., a novel Gossypium hirsutum L. photoperiod-sensitive male sterile mutant derived from CCRI 040029 using space mutation breeding technology） to evaluate heterosis. The F1 and F2 populations generated from the cross between CCRI 9106 and G. hirsutum L. cv. Letu 603 were used for genetic analyses. A bulked segregant analysis combined with simple sequence repeat （SSR）-based techniques were used to detect markers linked with the male sterility gene. An F2 population （186 plants） from the CCRI 9106 × Letu 603 cross was used for gene mapping analyses. [Results] All F1 plants were fertile, and the segregation ratio of fertility phenotypes in the F2 population was consistent with the 3：1 ratio of Mendelian inheritance. This indicated that male sterility was controlled by a recessive gene. Out of 16 544 S SRs, 18 were linked with the gene. The genetic linkage map suggested that the gene was located on chromosome D12 of the upland cotton genome withiv markers NAU3442 and CGR6339, with a 0.2-cM genetic distance to them. This photoperiod-sensitive male sterility gene was named ys-1. [Conclusion] The mapping results for ys-1 may be applicable for the fine-mapping and positional cloning of the photoperiod-sensitive male sterility gene.

关 键 词：陆地棉 光敏雄性不育 ys-1基因 隐性基因 基因定位 

分 类 号：S562[农业科学—作物学]