凝胶渗透色谱在高聚物分子量测定中的应用  

Application Development in Estimation of Molecular Weight by Gel Permeation Chromatography

作　　者：丁生龙[1] 丛湧[1] 高春梅[1] 柳明珠[1] Ding Shenglong Cong Yun Gao Chunmai Liu Mingzhu(College of Chemistry and Engineering, Lanzhou University, Lanzhou 73000)

机构地区：[1]兰州大学化学化工学院,兰州730000

出　　处：《化学通报》2017年第2期151-156,共6页Chemistry

摘　　要：本文综述了近年来凝胶渗透色谱在油溶性和水溶性高聚物分子量测定中的应用,着重分析测试条件对水溶性样品分子量的影响并归纳了解决的办法。同时,介绍了配备紫外可见和示差折光检测器、示差和光散射检测器后凝胶色谱的应用。此外,还概述了几种常见高聚物分子量的标定和校准方法,以便多种方法相互验证,得到较准确的分子量值。The application and development of molecular weight estimation for oils-soluble and water-soluble polymers by gel permeation chromatography （GPC） is summarized. The effects of pH, salt concentration and temperature on molecular weight estimation of water-soluble ones are presented. In addition, novel utilizations of GPC with dual detectors are introduced. Calibration methodologies in GPC, including various instrumental method and director calibration, as well as universal calibration are reviewed. With the combination of multi-detectors, the performance of one detector could be effectively checked against others and the estimated molecular weights of polymer tend to be reliable and precise.

关 键 词：凝胶渗透色谱 分子量 非体积效应 水溶性高分子 

分 类 号：O631.61[理学—高分子化学] O657.72[理学—化学]