电针预处理对急性心肌缺血再灌注损伤大鼠心肌组织水通道蛋白1表达及蛋白激酶C活性的影响  

Effects of electroacupuncture preconditioning on expression of aquaporin-1 and activity of protein kinase C in myocardium of rats with acute myocardial ischemia-reperfusion injury

作　　者：蔡荣林[1] 胡玲[1] 申国明[1] 余情[1] 王洁[1] 吴子建[1] 李梦[1] CAI Ronglin HU Ling SHEN Guoming YU Qing WANG Jie WU Zijian LI Meng(Institute of Acupuncture-Moxibustion and Meridian,Anhui University of CM,Hefei 230038, Chin)

机构地区：[1]安徽中医药大学针灸经络研究所,合肥230038

出　　处：《中国针灸》2017年第2期157-161,共5页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金项目:81303062;安徽省自然科学基金项目:1408085QH164;安徽省高校优秀青年人才支持计划重点项目:gxyqZD2016134;安徽高校科研创新平台建设项目:2015TD 033

摘　　要：目的:探讨电针"内关"与"太渊"穴预处理对急性心肌缺血再灌注损伤大鼠心肌的保护效应差异及部分作用机制。方法:96只Wistar大鼠随机分为假手术组、模型组、内关组、太渊组,每组24只。内关组与太渊组大鼠分别选取"内关"与"太渊"穴进行电针预处理,刺激电流1mA,频率2Hz,每次20min,每日1次,共干预7d。假手术组、模型组固定相同时间,不予电针干预。模型组、内关组、太渊组大鼠在末次电针24h后进行心肌缺血再灌注模型复制,假手术组在开胸后仅在相应部位用针空穿1次,不进行结扎。分别检测各组大鼠心肌缺血面积、梗死面积,心肌组织蛋白激酶C(protein kinase C,PKC)活性及水通道蛋白1(aquaporin 1,AQP1)表达情况。结果:模型组大鼠的心肌缺血面积、梗死面积、AQP1蛋白表达及PKC的活性显著升高,与假手术组比较差异均有统计学意义(均P<0.01),内关组大鼠的心肌缺血面积、梗死面积、PKC的活性和AQP1蛋白表达较模型组和太渊组均显著减少(P<0.01,P<0.05)。经Pearson相关分析,电针预处理后急性心肌缺血再灌注损伤大鼠心肌组织AQP1蛋白表达和PKC活性的变化呈显著正相关。结论:电针"内关"穴预处理可显著降低急性心肌缺血再灌注损伤大鼠心肌组织AQP1蛋白表达和PKC活性,其作用机制可能是通过抑制PKC活化进而抑制AQP1蛋白表达,从而发挥其心肌保护效应。Objective To explore the partial action mechanism and the myocardial protective effect differences between electroacupuncture（EA）preconditioning at＂Neiguan＂（PC 6）and＂Taiyuan＂（LU 9）in rats with acute myocardial ischemia-reperfusion injury.Methods Ninety-six Wistar rats were randomly assigned into a sham-operation group,a model group,a Neiguan group and a Taiyuan group,24 rats in each one.The rats in the Neiguan group and Taiyuan group were treated with EA（2Hz in frequency,1mA in intensity）at＂Neiguan＂（PC 6）and＂Taiyuan＂（LU 9）respectively,20 min per treatment,once a day for consecutive 7days.The rats in the shamoperation group and model group were treated with immobilization for the same time,and no EA was given.The model of myocardial ischemia-reperfusion injury was established in the model group,Neiguan group and Taiyuan group 24 hafter the end of EA,while the rats in the sham-operation group were treated with sham operation（no ligation was made during surgery）.The myocardial ischemic size,infarction size,activity of protein kinase C（PKC）and expression of aquaporin1（AQP1）in each group were detected.Results Compared with sham-operation group,the myocardial ischemic size,infarction size,AQP1 expression and PKC activity in the model group were significantly increased（all P〈0.01）;compared with the model group and Taiyuan group,the myocardial ischemic size,infarction size,PKC activity and AQP1 expression were significantly decreased in the Neiguan group（P〈0.01,P〈0.05）.By Pearson correlation analysis,the changes of AQP1 expression were positively correlated with those of PKC activity after EA preconditioning.Conclusion EA preconditioning at＂Neiguan＂（PC 6）couldsignificantly decrease myocardial AQP1 expression and PKC activity in rats with acute myocardial ischemia-reperfusion injuing,but the effect of EA preconditioning at＂Taiyuan＂（LU 9）is not obvious;its protective effect is likely to be achieved by inhibiting PKC activity and AQP1 expressi

关 键 词：急性心肌缺血再灌注损伤 电针预处理 水通道蛋白1 蛋白激酶C 

分 类 号：R246.1[医药卫生—针灸推拿学]