Chinese herbal medicine Yougui Pill reduces exogenous glucocorticoid-induced apoptosis in anterior pituitary cells  

作　　者：Yong-zhi Ji Long Geng Hong-bo Zhou Hua-chen Wei Hong-duo Chen 

机构地区：[1]Department of Dermatology,Second Hospital of Jilin University [2]Department of Dermatology,First Hospital of China Medical University [3]Department of Dermatology,Mount Sinai Medical Center

出　　处：《Neural Regeneration Research》2016年第12期1962-1968,共7页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.30471576,and 30872277;a grant from the Innovative Research Program in Universities of the Ministry of Education of China,No.IRT0760

摘　　要：Long-term glucocorticoid use may result in sustained suppression of one or more secreted components from the hypothalamo-pituitary-adrenal axis, and often results in apoptosis. Yougui Pill（YGP）, a 10-component traditional Chinese herbal medicine, has been shown to be clinically effective for glucocorticoid-induced suppression of the hypothalamo-pituitary-adrenal axis. However, the pharmacological and molecular mechanisms remain unclear. We hypothesized that YGP would exert an anti-apoptosis effect on dexamethasone-treated anterior pituitary cells. In vivo experiments showed that YGP significantly reduced the number of apoptotic cells, down-regulated m RNA expression of cytochrome c, caspase-3, and caspase-9, and up-regulated m RNA expression of Bcl-2. These findings suggest that YGP reduced glucocorticoid-induced apoptosis in rat anterior pituitary cells by regulating the mitochondria-mediated apoptosis pathway.Long-term glucocorticoid use may result in sustained suppression of one or more secreted components from the hypothalamo-pituitary-adrenal axis, and often results in apoptosis. Yougui Pill（YGP）, a 10-component traditional Chinese herbal medicine, has been shown to be clinically effective for glucocorticoid-induced suppression of the hypothalamo-pituitary-adrenal axis. However, the pharmacological and molecular mechanisms remain unclear. We hypothesized that YGP would exert an anti-apoptosis effect on dexamethasone-treated anterior pituitary cells. In vivo experiments showed that YGP significantly reduced the number of apoptotic cells, down-regulated m RNA expression of cytochrome c, caspase-3, and caspase-9, and up-regulated m RNA expression of Bcl-2. These findings suggest that YGP reduced glucocorticoid-induced apoptosis in rat anterior pituitary cells by regulating the mitochondria-mediated apoptosis pathway.

关 键 词：nerve regeneration Yougui Pill glucocorticoid apoptosis Bcl-2 cytochrome c caspase-3 caspase-9 mitochondrial membrane potential neural regeneration 

分 类 号：R285[医药卫生—中药学]