枸杞根腐病病原菌的快速分离及培养方法  

Study on Fast Separation and Cultural Method of Pathogenic Bacteria of Wolfberry Root Rot

作　　者：田晓凤[1] 张波[2] 罗青[2] 李云翔[2] 

机构地区：[1]北方民族大学,宁夏银川750021 [2]宁夏农林科学院,宁夏银川750002

出　　处：《宁夏农林科技》2016年第10期37-38,F0002,共3页Journal of Ningxia Agriculture and Forestry Science and Technology

基　　金：宁夏农林科学院科技创新先导项目--枸杞根腐病高抗砧木筛选与苗期嫁接技术研究(NKYJ-16-23)

摘　　要：本文探索了枸杞根腐病病原菌的快速分离及培养方法。结果表明,为了保证在无杂菌、无污染前提下快速分离出枸杞根腐病病原菌,可选用新鲜病组织,先用75%酒精消毒10 m in,再用0.1%升汞消毒10 m in,以PD A为培养基,28℃恒温培养,培养成功率高。Fast separation and cultural method of pathogenic bacteria of wolfberry root rot were discussed in this paper. The results showed that in order to get fast separation of pathogenic bacteria of wolfberry root rot without infectious microbe and contamination, fresh diseased tissues could be used. Disinfect for 10 minutes with 75% ethyl alcohol content and another 10 minutes with 0.1% mercury, then cultivate under 28 ℃ with PDA as medium. Though this way can people have the highest successful cultivation rate.

关 键 词：根腐病 病原菌 培养条件 

分 类 号：S435.67[农业科学—农业昆虫与害虫防治]