健心颗粒对慢性心力衰竭大鼠CaN mRNA、UⅡRNA表达的影响  

Effects of Jianxin Granules on the Expressions of Calcineurin mRNA and Urotensin Ⅱ RNA in Rats with Chronic Heart Failure

作　　者：王永[1] 施丹[1] 蔡琦霞 李志朋[1] 李娥[1] 陈美华[1] 

机构地区：[1]福建中医药大学附属第二人民医院,福州350001

出　　处：《中西医结合心脑血管病杂志》2017年第2期158-161,共4页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：福建省卫生和计划生育委员会中医处课题(No.wzln201304)

摘　　要：目的探讨健心颗粒不同剂量对慢性心力衰竭大鼠钙调神经磷酸酶(Ca N)mRNA、尾加压素Ⅱ(UⅡ)RNA表达的影响。方法将18周龄的自发性高血压大鼠64只随机分为培哚普利组、健心颗粒等剂量组、健心颗粒高剂量组、阴性对照组,每组16只。培哚普利组培哚普利0.36 mg/(kg·d)灌胃、健心颗粒等剂量组、健心颗粒高剂量组分别以健心颗粒3.2 g/(kg·d)、6.1 g/(kg·d)灌胃;阴性对照组予生理盐水,按1 m L/100 g灌胃给药,每日两次。给药后第21天、第35天各组分别处死8只大鼠,采用荧光定量PCR检测心室肌组织Ca N mRNA、UⅡRNA表达。结果第21天与阴性对照组比较,培哚普利组Ca N mRNA,健心颗粒等剂量组、健心颗粒高剂量组大鼠心室肌组织Ca N mRNA、UⅡRNA表达均显著下降,且健心颗粒高剂量组明显低于健心颗粒等剂量组,差异有统计学意义(P<0.05);第35天,与阴性对照组比较,其他各组大鼠心室肌组织Ca N mRNA、UⅡRNA的表达均显著下降,且培哚普利组<健心颗粒等剂量组<健心颗粒高剂量组,差异有统计学意义(P<0.05)。结论健心颗粒能减少UⅡ、Ca N基因表达,可显著抑制心室重构。Objective To investigate the effects of Jianxin granules（ JXG） on the expressions of calcineurin（ Ca N） mRNA and urotensin Ⅱ（ UⅡ） RNA in rats with chronic heart failure（ CHF）. Methods Sixty-four 18-week-old spontaneously hypertensive rats（ SHR） were randomly divided into 4 groups： perindopril group（ n = 16） intragastric administrated by perindopril 0.36 mg /（ kg·d）,JXG low dose group（ n =16） intragastric administrated by 3.2 g/（ kg·d）,JXG high dose group（ n =16） intragastric administrated by JXG 6.4 g/（ kg·d）,and negative control group（ n = 16） given saline solution. After administration of the 21 st day,35 th day,8 rats in each group were sacrificed. The expressions of Ca N mRNA and UⅡ RNA were detected by fluorescence quantitative PCR. Results Compared with negative control group,the expressions of Ca N mRNA and UⅡ RNA significantly declined at 21 st day in JXG groups,which was lower in JXG high dose group than that in JXG low dose group（ P〈 0.05）. Compared with negative control group,the expressions of Ca N mRNA and UⅡ RNA significantly declined at 35 th day in JXG groups and perindopril group,which was lower in JXG groups than that in perindopril group,and was lower in JXG high dose group than that in JXG low dose group（ P〈 0.05）. Conclusion JXG can reduce the expressions of Ca N mRNA and UⅡ RNA,and inhibit ventricular remodeling in SHR.

关 键 词：慢性心力衰竭 健心颗粒 自发性高血压大鼠 钙调神经磷酸酶 尾加压素Ⅱ 

分 类 号：R285.5[医药卫生—中药学]