金娃娃萱草的离体培养及植株再生  

In vitro Culture and Plantlet Regeneration of Hemerocallis fuava

作　　者：张晓军[1] 孙平平[1] 于凤超[1] 郝婧玮[1] 宗宪春[1] 魏继承[1] 孙晓薇[1] 柴军红[1] 纪春艳[1] 

机构地区：[1]牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157011

出　　处：《牡丹江师范学院学报（自然科学版）》2017年第1期49-51,共3页Journal of Mudanjiang Normal University：Natural Sciences Edition

基　　金：国家烟草专卖局基因组重大专项[110201501015(JY-02)];牡丹江师范学院省级重点创新预研项目(SY201229);牡丹江市科学技术计划项目(Z2013n020)

摘　　要：研究金娃娃萱草的组织培养及植株再生.以MS为基本培养基,离体培养金娃娃萱草新生侧苗的根状茎.研究结果表明:芽诱导的最佳培养为MS+6-BA 0.5mg·L^(-1)+NAA 0.2mg·L^(-1),芽诱导效率可达100%;最佳生根培养基为加入0.2 mg·L^(-1)NAA的1/2MS培养基,生根率达100%,且根系发达健壮,茎叶生长旺盛.To study In vitro Culture and Plantlet Regeneration of Hemerocallis fuava. Root-like stem explant was cultured in vitro on MS medium. The results showed that the optimum medium of inducing cluster buds was MS ＋ 6-BA 0. 5mg . L-1＋NAA 0. 2 mg . L-1 ,the efficiency of in-ducing buds was 100 %. The 1/2 MS medium with addition of 0. 2 mg . L-1 NAA was the most appropriate for inducing test-tube plantlet to produce roots and the rate of rooting might reach 100 % ,and the root system developed robust, stem and leaf grew well.

关 键 词：金娃娃萱草 组织离体培养 植株再生 

分 类 号：Q338[生物学—遗传学]