基于PC12细胞的西维因神经损伤作用机理研究  

Mechanism of Neurotoxicity Injuries Induced by Carbaryl in PC12 Cells

作　　者：徐梦蕾[1] 王新欣[1] 刘静波[1] 高宇[2] 熊晋锋 赵颂宁[1] 黄延军[1] 

机构地区：[1]吉林大学营养与功能食品实验室,吉林长春130062 [2]吉林农业大学农学院,吉林长春130118 [3]长春生物制品研究所有限责任公司,吉林长春130012

出　　处：《现代食品科技》2016年第12期1-5,65,共6页Modern Food Science and Technology

基　　金：国家科技支撑计划项目(2012BAD33B03);吉林省世行贷款农产品质量安全项目(2011-Z67);吉林大学研究生创新基金资助项目(2015052);吉林农业大学科研启动基金资助项目(2015010);国家级大学生创新创业训练计划项目(201510193002)

摘　　要：本文以PC12细胞为神经细胞模型,研究了西维因对神经细胞的损伤作用机理。当PC12细胞暴露于0.00μg/m L^200.00μg/m L不同浓度的西维因溶液时,表现出了一系列显著变化。随着西维因溶液浓度的增大,细胞的存活率从(100.00±0.46)%下降到了(27.86±1.69)%;细胞内乳酸脱氢酶(LDH)漏出率增多;丙二醛(MDA)含量从19.27±0.96 nmol/mg prot逐渐增加到112.39±1.59nmol/mg prot;超氧化物歧化酶(SOD)活力从4.08±0.87 U/mg prot增强到17.77±0.43 U/mg prot,同时其抑制率也从(19.00±1.66)%增加到了(73.00±1.71)%;同时谷胱甘肽(GSH)含量从96.15±6.10μmol/g prot逐渐减少到22.91±3.98μmol/g prot;细胞相对荧光强度(RFI)从37.38±11.48先增强到45.56±11.96,后因细胞严重损伤又减弱到17.11±1.50。细胞液内乙酰胆碱(ACh)含量也逐渐增加。同时,利用激光共聚焦扫描显微镜观察到了线粒体膜电位的显著下降。西维因对PC12细胞表现出很强的神经毒性。The present study was planned to evaluate neurotoxicity injuries induced by carbaryl in PC12 cells, which were used as a model for nervous cell system. In this study, PC12 cells were exposed to carbaryl in different concentrations from 0.00 μg/m L to 200.00 μg/m L, and showed significant changes. Cells viability rates decreased from（100.00±0.46）% to（27.86±1.69）% with a increase of carbaryl concentration. Carbaryl treatment resulted in a significant increase in the levels of LDH leakage rate. MDA content also increased from 19.27±0.96 nmol/mg·prot to 112.39±1.59 nmol/mg prot. SOD activity increased from 4.08±0.87 U/mg prot to 17.77±0.43 U/mg prot, and SOD inhibition rate increased from（19.00±1.66）% to（73.00±1.71）%, however, GSH content decreased from 96.15±6.10 μmol/g prot to 22.91±3.98 μmol/g prot. RFI increased from 37.38±11.48 to 45.56±11.96 firstly, then decreased to 17.11±1.50 with the damage of cells. Ach in medium increased by carbaryl treatment. A significantly decrease in integrity of mitochondrial membrane potential was observed by laser scanning confocal microscope. Carbaryl can induce neurotoxicity in PC12 cells.

关 键 词：PC12细胞 西维因 损伤 细胞毒性 神经毒性 

分 类 号：R114[医药卫生—卫生毒理学]