基于高通量培养的胶球藻C-169生物量和油脂积累能力的快速评价  

Rapid Evaluation of Biomass and Lipid Accumulation Capacity in Coccomyxa subellipsoidea C-169 Cultured in a High-throughput System

作　　者：魏东[1] 李露[1] 

机构地区：[1]华南理工大学食品科学与工程学院,广东广州510640

出　　处：《现代食品科技》2016年第12期113-119,共7页Modern Food Science and Technology

基　　金：国家自然科学基金项目(31370383);国家863计划项目(2013AA065802);广东省海洋渔业科技与产业发展专项(A201401C01);广东省公益研究与能力建设项目(2015A020216003)

摘　　要：本研究利用CO2培养箱内48孔板振荡光照培养专利设计的微藻高通量培养体系,结合细胞组学技术,系统评价了氮源和盐度梯度下胶球藻C-169细胞的生长速率、生物量浓度和油脂积累能力。结果表明,该培养体系的均一性和稳定性良好,可实现微藻的高通量及多处理培养。富氮培养基可显著提高胶球藻C-169的细胞生长速率和生物量浓度,缺氮培养基显著驱动了细胞积累中性脂。在含1/4氮的培养基中藻细胞的生物量浓度是4倍氮培养基中的25%,而中性脂含量（平均荧光强度表示）则高达后者的16倍以上。在015‰盐度范围内胶球藻C-169的生长状况良好且无显著差异（p〉0.05）,15‰以上盐度藻细胞生长受到明显抑制。10‰盐度下中性脂含量（以平均荧光强度表示）显著高于所有其他组（p〈0.05）,说明该盐度有利于驱动细胞积累中性脂。Coccomyxa subellipsoidea C-169 cells were cultured using a high-throughput microalgal culture system with a patented design, 48-well microplate with shaking and illumination in a CO2 incubator. Combined with cytomics technology, the growth rate, biomass concentration, and lipid accumulation capacity in C.subellipsoidea C-169 cells under nitrogen and salinity gradients were systematically evaluated. The results showed that the culture system had excellent uniformity and stability, and could achieve high-throughput and multi-treatment microalgae culture. Cell growth and biomass concentration were significantly enhanced in nitrogen-rich medium; whereas neutral lipid accumulation was significantly enhanced in nitrogen-deficient medium. Biomass concentration in the medium containing 1/4 nitrogen concentration was 25% of that in medium containing four times as much nitrogen; however, neutral lipid content（shown as average fluorescence intensity） was more than 16 times higher. At salinity levels of 015%, C. subellipsoidea C-169 cells grew well, but rates were not significantly different（p〉0.05）. However, cell growth was significantly inhibited when salinity was higher than 15%. The neutral lipid content（shown as average fluorescence intensity） at a salinity of 10% was significantly higher than that of all other groups（p〈0.05）, indicating that this salinity level could facilitate accumulation of neutral lipids.

关 键 词：胶球藻C-169 高通量 氮源 盐度 细胞生长 

分 类 号：Q946[生物学—植物学] TE667[石油与天然气工程—油气加工工程]