银杏提取物EGb761对小鼠肺腺癌血管生成及VEGF、TSP-1表达的影响  

Effects of Ginkgo biloba extract EGb761 in angiogenesis to lung adenocarcinoma mice and the expression of VEGF and TSP-1

作　　者：衷敬华[1] 徐房添[1] 许明君[1] 王祥财[1] 王钇力[1] 刘霞[1] 杨静[1] 廖家华[1] 张积仁[2] 

机构地区：[1]赣南医学院第一附属医院肿瘤科,赣州341000 [2]南方医科大学珠江医院肿瘤中心,广州510282

出　　处：《江西医药》2016年第12期1344-1346,1349,共4页Jiangxi Medical Journal

基　　金：江西省卫计委中药局基金支持(2011Z0017)

摘　　要：目的观察银杏提取物EGb761对小鼠肺腺癌血管生成的影响。方法构建小鼠肺腺癌模型,用银杏提取物EGb761腹腔注射给药,在10d、20d、30d、40d观察小鼠的整体状态,计算肺组织肿瘤转移结节数目、瘤重及抑瘤率,检测肿瘤组织中血管内皮生长因子(VEGF)、肿瘤血管生长(MVD)、凝血酶敏感蛋白-1(TSP-1)的表达。结果 EGb761各剂量组的瘤重及肺转移灶数目均低于模型组,EGb761各剂量组VEGF表达、肿瘤血管密度均低于模型组,EGb761各组TSP-1显著增高,均具有显著性差异(P<0.05)。结论在小鼠肺腺癌模型中,银杏提取物EGb761可抑制小鼠肺腺癌肿瘤血管生成,其机制可能是通过调节VEGF表达减少,TSP-1表达增高而达到的。Objective To observe EGb761 effects on lung adenocarcinoma angiogenesis in mice. Methods To build the mice model of lung adenocarcinoma,administered by intraperitoneal injection of EGb761,in 10 day,20 day,30 day,40 day to observe the general condition of mice,calculation of tumor weight,inhibitory rate and lung metastatic nodules,detection expression of tumor tissue of vascular endothelial growth factor（VEGF） and tumor blood vessel growth（MVD）,thrombin sensitive protein-1（TSP-1）.Results Tumor weight and number of pulmonary metastases each dose group of EGb761 were lower than that of model group,VEGF expression and tumor vascular density of each dose group of EGb761 were lower than the model group. EGb761 groups significantly higher TSP-1,have significant difference（P0.05）. Conclusion In lung adenocarcinoma in mice model,EGb761 can significantly inhibit the growth of lung adenocarcinoma,its mechanism may be through reducing the expression of VEGF,improve the expression of TSP-1 to inhibit angiogenesis and tumor nutrition supply,thus affecting tumor growth.

关 键 词：银杏提取物EGb761 小鼠肺腺癌模型 肿瘤血管生长(MVD) 血管内皮生长因子(VEGF) 凝血酶敏感蛋白-1(TSP-1) 

分 类 号：R273[医药卫生—中西医结合]