南京市大气PM_(2.5)急性暴露对小鼠心肺组织的氧化损伤研究  

Oxidative damage due to acute exposure to PM_(2.5) in air from Nanjing in heart and lung tissues of mice

作　　者：孙一轩 王金花[1] 钱新[1] 李慧明[1] 王呈[1] 

机构地区：[1]南京大学环境学院污染控制与资源化研究国家重点实验室,江苏南京210023

出　　处：《环境与健康杂志》2016年第10期847-850,共4页Journal of Environment and Health

基　　金：国家自然科学基金(41501549;41271511)

摘　　要：目的探讨大气PM_(2.5)引起的小鼠心肺组织的氧化损伤。方法将24只健康6周龄SPF级C57BL/6雄性小鼠按体重随机分为4组,分别为空白对照(无菌生理盐水)组和1.5、7.5、37.5 mg/kg PM_(2.5)染毒组,每组6只。染毒采用一次性气管注入法,染毒容量为1.5 ml/kg。染毒24 h后,检测小鼠心、肺组织超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力以及谷胱甘肽(GSH)和丙二醛(MDA)含量。同时,分析PM_(2.5)主要化学组分。结果所采集的PM_(2.5)样品主要化学组分浓度占比分别为有机物(24.8%)、元素碳(4.94%)、SO_4^(2-)(12.2%)、NO_3^-(15.3%)、NH_4^+(2.39%)、矿物尘(8.09%)、金属元素(1.61%)。与对照组比较,37.5 mg/kg PM_(2.5)染毒组小鼠心组织MDA含量较高,且CAT、SOD活力较低;而7.5、37.5 mg/kg PM_(2.5)染毒组小鼠心组织GSH含量均较低,差异均有统计学意义(P<0.05)。与对照组比较,7.5、37.5 mg/kg PM_(2.5)染毒组小鼠肺组织MDA含量均较高,差异均有统计学意义(P<0.05);而各剂量PM_(2.5)染毒组小鼠肺组织GSH的含量及CAT、SOD的活力均无明显改变。结论高浓度PM_(2.5)急性暴露可诱发小鼠心、肺组织的氧化应激反应。Objective To investigate the oxidative damage caused by PM_（2.5） acute exposure in hearts and lungs of mice.Methods According to body weight,24 healthy C57BL/6（SPF） male mice were randomly divided into the control（normal saline） group and three treatment groups which were treated with suspension mixture at the dosage of 1.5,7.5 and 37.5 mg/kg body weight,respectively through intratracheal instillation. Each mouse was injected with 1.5 ml/kg body weight of the suspension mixture. 24-hour after exposure,the heart and lung tissues were collected for the determination of activities of superoxide dismutase（SOD） and catalase（CAT） as well as the level of glutathione（GSH） and malondialdehyde（MDA）. PM_（2.5） composition was analyzed also. Results The chemical components of organic matters,elemental carbon,SO_4^（2-）,NO_3^-,NH_4~＋,mineral dust and metallic element contributed 24.8%,4.94%,12.2%,15.3%,2.39%,8.09% and 1.61% respectively to the PM_（2.5） mass. Compared with the control group,there was a significant increase of the MDA contents（P〈0.05） and a significant decrease of the activities of CAT and SOD（P〈0.05） in heart tissues of mice in 37.5 mg/kg PM_（2.5） group. Compared with the control group,the levels of MDA in lung tissues of mice in 7.5,37.5 mg/kg PM_（2.5） groups increased significantly（P〈0.05）.However,no significant differences were found in GSH contents and the activities of CAT and SOD among the four groups.Conclusion Acute exposure to airborne fine particles with higher concentrations can cause severe oxidative stress in heart and lung tissues of mice.

关 键 词：PM2.5 心 肺 氧化损伤 抗氧化酶 

分 类 号：R994.6[医药卫生—毒理学]