Potential of capillary electrophoresis mass spectrometry for the characterization and monitoring of amine-derivatized naphthenic acids from oil sands process-affected water  

作　　者：Matthew S.MacLennan Cai Tie Kevin Kovalchik Kerry M.Peru Xinxiang Zhang John V.Headley David D.Y.Chen 

机构地区：[1]Department of Chemistry, University of British Columbia, Vancouver, BC V6T 1Z1, Canada. [2]State Key Laboratory of Bioactiue Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College &Chinese Academy of Medical Sciences, Beijing 100050, China [3]Water Science and Technology Directorate, Science and Technology Branch, Environment and Climate Change Canada, Saskatoon,SK S7N 3H5, Canada [4]College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China

出　　处：《Journal of Environmental Sciences》2016年第11期203-212,共10页环境科学学报（英文版）

基　　金：supported by the Environment Canada and the NSERC

摘　　要：Capillary electrophoresis coupled to mass spectrometry（CE–MS） was used for the analysis of naphthenic acid fraction compounds（NAFCs） of oil sands process-affected water（OSPW）. A standard mixture of amine-derivatized naphthenic acids is injected directly onto the CE column and analyzed by CE–MS in less than 15 min. Time of flight MS analysis（TOFMS）, optimized for high molecular weight ions, showed NAFCs between 250 and 800 m/z. With a quadrupole mass analyzer, only low-molecular weight NAFCs（between 100 and 450 m/z） are visible under our experimental conditions. Derivatization of NAFCs consisted of two-step amidation reactions mediated by 1-ethyl-3-（3-dimethylaminopropyl）carbodiimide（EDC）, or mediated by a mixture of EDC and N-hydroxysuccinimide, in dimethyl sulfoxide, dichloromethane or ethyl acetate. The optimum background electrolyte composition was determined to be 30%（V/V） methanol in water and 2%（V/V） formic acid. NAFCs extracted from OSPW in the Athabasca oil sands region were used to demonstrate the feasibility of CE–MS for the analysis of NAFCs in environmental samples, showing that the labeled naphthenic acids are in the mass range of 350 to 1500 m/z.Capillary electrophoresis coupled to mass spectrometry（CE–MS） was used for the analysis of naphthenic acid fraction compounds（NAFCs） of oil sands process-affected water（OSPW）. A standard mixture of amine-derivatized naphthenic acids is injected directly onto the CE column and analyzed by CE–MS in less than 15 min. Time of flight MS analysis（TOFMS）, optimized for high molecular weight ions, showed NAFCs between 250 and 800 m/z. With a quadrupole mass analyzer, only low-molecular weight NAFCs（between 100 and 450 m/z） are visible under our experimental conditions. Derivatization of NAFCs consisted of two-step amidation reactions mediated by 1-ethyl-3-（3-dimethylaminopropyl）carbodiimide（EDC）, or mediated by a mixture of EDC and N-hydroxysuccinimide, in dimethyl sulfoxide, dichloromethane or ethyl acetate. The optimum background electrolyte composition was determined to be 30%（V/V） methanol in water and 2%（V/V） formic acid. NAFCs extracted from OSPW in the Athabasca oil sands region were used to demonstrate the feasibility of CE–MS for the analysis of NAFCs in environmental samples, showing that the labeled naphthenic acids are in the mass range of 350 to 1500 m/z.

关 键 词：Capillary electrophoresis Mass spectrometry Electrospray ionization Carbodiimide Oil sands process-affected water Naphthenic acids 

分 类 号：O657.63[理学—分析化学] X832[理学—化学]