超声-酶法提取两面针中血根碱的工艺优选  

Technology Optimization of Ultrasonic-enzyme-assisted Extraction of Sanguinarine from Zanthoxylum nitidum

作　　者：陆世惠[1] 陈冉[2] 林瑶[1] 陈楠[1] 朱晓莹[2] 

机构地区：[1]右江民族医学院药学院,百色533000 [2]右江民族医学院科学实验中心,百色533000

出　　处：《医药导报》2017年第2期193-196,共4页Herald of Medicine

基　　金：广西自然科学基金资助项目(2014GXNSFBA118185)

摘　　要：目的优化超声-酶法提取两面针中血根碱的工艺条件。方法以高效液相色谱(HPLC)法测定血根碱提取率为指标,考察提取溶剂加酸和酶解预处理对两面针超声提取的适用性,正交实验优化超声功率、提取次数和溶剂量,最后动态过程优化超声提取时间。结果最佳工艺条件为:两面针复合酶预处理后,以体积分数40%乙醇(含盐酸0.2%)超声(250 W)提取3次,第1次以6倍量溶剂提取15 min,第2,3次各以3倍量溶剂提取12 min,血根碱提取率为88.6%。结论该提取工艺经济、高效、节能、省时,可为开发利用血根碱提供实验基础。ObjectiveTo optimize the technology conditions of ultrasonic-enzyme-assisted extraction for sanguinarine from Zanthoxylum nitidum. MethodsExtraction rate of sanguinarine determined by HPLC was served as an index. The applicability of the extraction solvent added with acid and enzymatic hydrolysis pretreatment to the ultrasonic-enzyme-assisted extraction of Zanthoxylum nitidum was investigated. Ultrasonic power, extraction frequency and solvent volume were optimized by orthogonal experiment. Finally, ultrasonic extraction time was optimized in dynamic process. ResultsThe optimal process was as follows： Zanthoxylum nitidum powder was extracted 3 times by ultrasonic-wave （250 W） with 40% ethanol （0.2% hydrochloride） as solvent （extracted for 15 min with 6-fold solvent at the first time, then extracted for 12 min with 3-fold solvent at the second and the third time, respectively）. The extraction rate of sanguinarine was 88.6%. ConclusionThe process is economic, efficient, energy- and time-saving, and provides experimental basis for industrial production of sanguinarine.

关 键 词：两面针 血根碱 超声-酶法 提取工艺 

分 类 号：R284.2[医药卫生—中药学]