两种人NK细胞培养体系相关性研究  

The relationship between small-scale research culture system and clinical grade preparative culture system in generating of human natural killer cells

作　　者：陈伟[1] 欧阳明玥 蔺迪[1] 刘军德[1] 杨冀[1] 薛峰[1] 谢晓冬[2] 于卉影[1] CHEN Wei OUYANG Ming-yue LIN Di LIU Jun-de YANG Ji XUE Feng XIE Xiao-dong YU I-lui-ying(Department of Experimental Medicine, Cancer Center of PLA, The General Hospital of Shenyang Military Command, Shenyang 110016, China)

机构地区：[1]沈阳军区总医院全军肿瘤诊治中心医学实验科,辽宁沈阳110016 [2]沈阳军区总医院全军肿瘤诊治中心肿瘤科,辽宁沈阳110016

出　　处：《临床军医杂志》2016年第12期1272-1275,共4页Clinical Journal of Medical Officers

摘　　要：目的探讨人自然杀伤(NK)细胞小规模研究型培养体系与大规模临床制备型培养体系的相关性。方法分离11例健康志愿者的外周血,制备外周血单个核细胞(PBMCs),分别于小规模研究型培养体系与大规模临床制备型培养体系中进行诱导和扩增。比较两种培养体系下,人NK细胞的增殖倍数、细胞表型、相关活化受体以及细胞毒作用。结果第14天,两种培养体系下人NK细胞的增殖倍数、细胞表型以及细胞毒作用的方面比较,差异均无统计学意义(P>0.05)。结论人NK细胞小规模研究型培养体系和临床制备型培养体系具有较好的一致性,前者可为后者的建立与优化提供稳定的研究模型,且能明显降低研究成本。Objective To explore the comparative relationship between small-scale research culture system and clinical grade preparative culture system in generating of human natural killer（ NK） cells. Methods Peripheral blood mononuclear cells（ PBMCs） isolated from 11 healthy volunteers were induced and amplified in the above two NK cells culture systems in vitro. Multiples of NK cell proliferation,cell phenotype,activating receptor and cytotoxicity were analyzed. Results At the end of culture（ day 14）,there were no significant differences in cell proliferation,cell phenotype and cytotoxicity between the two NK cell culture systems（ P 0. 05）.Conclusion Small-scale research culture system can be used as a more cost-effective stable research model for establishment and optimization of human NK cell production in lieu of a clinical grade preparative culture system.

关 键 词：自然杀伤细胞 免疫治疗 细胞培养 肿瘤 

分 类 号：R392.1[医药卫生—免疫学]