基于液相色谱/四极杆飞行时间质谱的产ATPA的芽胞杆菌筛选  

Screening of Bacillus Strains Producing ATPA Based on Liquid Chromatography-Hybrid Quadrupole Time-of-Flight Mass Spectrometry

作　　者：陈德局[1] 陈峥[1] 张海峰[1] 陈小强[1] 朱育菁[1] 刘波[1] CHEN Deju CHEN Zheng ZHANG Haifeng CHEN Xiaoqiang ZHU YuJing LIU Bo(Institute of Agrobiological Rsources, Fujian Academy of Agricultural Sciences, Fuzhou 350003, Fujian, Chin)

机构地区：[1]福建省农业科学院农业生物资源研究所,福建福州350003

出　　处：《氨基酸和生物资源》2016年第4期37-41,共5页Amino Acids & Biotic Resources

基　　金：福建省重大专项项目农业科技重大专项<果蔬微生物保鲜加工增值关键技术的研究与应用>(2015NZ0003);福建省农业科学院科技创新团队PI项目(2016PI-43);福建省省属公益项目(2016R1017-6);福建省农科院英才计划(YC2016-14)

摘　　要：本文采用液相-四级杆飞行时间质谱的方法筛选产2-amino-3-(3-hydroxy-5-tert-butylisoxazol-4-yl)propanoic acid(ATPA)的芽胞杆菌菌株。通过分析50株芽胞杆菌菌株发酵液中胞外化合物成分,筛选出合成ATPA的2株芽胞杆菌,为嗜碱芽胞杆菌(Bacillus alcalophilus)FJAT-10014和栗褐芽胞杆菌(Bacillus plakortidis)FJAT-8757,该成分在发酵液中的相对含量分别为1.43%和1.28%,ATPA与色谱库检索得分(score)分别为93.41和95.01。To screen the Bacillus strains producing ATPA, extracellular metabolites of 50 Bacillus strains were analyzed by liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry （LC-QTOFMS）. The result showed that the relative contents of ATPA were 1.43 % and 1.28% in Bacillus plakortidis FJAT-8757 and Bacillus alcalophilus FJAT-10014, respectively, and the score of specialized m/z matched to ATPA m/z was 93.41 and 95.01 in FJAT-8757 and FJAT-10014, respectively.

关 键 词：液相色谱/四极杆飞行时间质谱(LC-QTOF MS) 芽胞杆菌 α-氨基-3羟基-5-甲基-4-异恶唑丙酸(ATPA) 红藻氨酸受体(KARs) 

分 类 号：Q9[生物学]