重组5型腺病毒处理不会加重卵清蛋白诱发哮喘小鼠的哮喘症状  

Recombinant adenovirus type 5 does not aggravate asthma symptoms in a mouse model of ovalbumin-induced asthma

作　　者：张正玲[1] 张翊玲[2] 韩婧[2] 路苹[2] 徐琳[2] 

机构地区：[1]遵义师范学院生命科学学院,贵州遵义563002 [2]贵州省人民医院呼吸内科,国家呼吸疾病临床医学研究中心核心单位,贵州贵阳550002

出　　处：《细胞与分子免疫学杂志》2016年第12期1590-1594,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：贵州省科技厅联合基金[黔科合IH字(2015)7034号,黔科合SY(2010)3124号]

摘　　要：目的探索重组5型腺病毒（rAd5）对卵清蛋白（OVA）诱发的小鼠哮喘模型的影响。方法 60只6~8周龄、体质量18~20 g的雌性C57BL/6小鼠被随机分为健康对照组、rAd5对照组、OVA哮喘对照组、rAd5处理的OVA哮喘组,每组15只。OVA哮喘对照组、rAd5处理的OVA哮喘组在第0、7、14天分别采用含50μg OVA和2 mg氢氧化铝混悬液腹腔注射致敏3次、于第42、43、44天连续3 d用OVA雾化激发制作哮喘模型。其余两组采用相同体积的生理盐水处理。rAd5对照组、rAd5处理的OVA哮喘组于实验第21天开始肌注rAd5（2.5×109个病毒颗粒/只）1次。并于第35天滴鼻加强1次rAd5（2.5×109个病毒颗粒/只）。所有小鼠于最后一次雾化激发48 h内进行安乐死。通过无创肺功能仪体描法检测小鼠气道高反应,收集血清及肺泡灌洗液标本、收集肺组织用于HE及PAS染色,通过ELISA检测肺泡灌洗液中的白细胞介素4（IL-4）、IL-5、IL-13及血清中的总IgE。结果与健康对照组及rAd5对照组相比,OVA哮喘对照组、rAd5处理的OVA哮喘组其肺部炎症及黏液分泌显著增加,其肺泡灌洗液中IL-4、IL-5、IL-13及血清中的总IgE含量显著增高,其气道高反应显著升高。然而,OVA哮喘对照组与rAd5处理的OVA哮喘组之间无显著差异。结论重组5型腺病毒作用于小鼠哮喘模型不会加重哮喘症状。Objective To explore the effect of recombinant adenovirus type 5 （rAd5） on a mouse model of ovalbumin （OVA）-induced asthma. Methods Sixty female C57BL/6 mice （6 -8 weeks old and 18 -20 g） were randomly divided into 4 groups： healthy control group, rAd5 control group, OVA asthma control group and OVA asthma group with fAdS immunization. 15 mice in each group. The mice in the OVA asthma control group and the OVA asthma group with rAcl5 immunization were sensitized by intraperitoneal injection of 50 μg OVA emulsified in 2 mg of aluminum potassium sulfate on day O, 7 and 14; subsequently, the mice in the two groups were exposed to OVA aerosol challenge for 3 consecutive days （ on day 42, 43 and 44）. Meanwhile, mice in the healthy control group and the rAd5 control group were given an equal volume of norrna] saline. What＇s mare, mice in the OVA asthma group with rAd5 immunization and the rAclS control group were immunized intramuscularly with 2.5 × 109 viral particles （VP） of rAd5 vectors on day 21, and were boosted intranasally with the same dose Of rAd5 vectors on day 35. All mice were euthanized within 48 hours after the last challenge. The airway hyper-responsiveness （AHR） of mice was measured by single-chamber, whole-body plethysmography. The blood was obtained, the samples of bronchoalveolar lavage fluid （BALF） were collected, and lung tissues were prepared for HE and PAS staining. The levels of interleukin-4 （IL-4）, IL-5, IL-13 in BALF and total IgE in serum were detected by ELISA. Results Compared with the healthy control group and the tAd5 control group, the pulmonary inflammation and mucus production significantly increased in the OVA asthma control group and the OVA asthma group with rAd5 immunization. The levels of IL-4, IL-5, IL-13 in BALF, total serum IgE and the AHR were also significantly enhanced in the OVA asthma control group and the OVA asthma group with rAd5 immunization. However, no significant difference was showed between the OVA asthma control group and th

关 键 词：小鼠 哮喘 重组5型腺病毒 卵清蛋白 

分 类 号：R256.12[医药卫生—中医内科学] R511.8[医药卫生—中医学]