白细胞介素22对新生SD大鼠高氧肺损伤有保护作用  

Protective effect of interleukin 22 against hyperoxia-induced lung injury in neonatal rats

作　　者：袁利[1] 刘秀香[1] 高瑞伟 马金帅 陆素妮 张海鸿[1] 

机构地区：[1]滨州医学院附属医院新生儿科,山东滨州256600

出　　处：《细胞与分子免疫学杂志》2016年第12期1623-1626,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：山东省医药卫生科技发展计划(2015WS0493);山东省科学技术发展计划(BY2009KJ15)

摘　　要：目的探讨白细胞介素22(IL-22)对SD新生大鼠高氧肺损伤的保护作用。方法新生SD大鼠随机分为对照组、高氧组和IL-22(10 ng/g)治疗组,每组随机分成1、3、7 d三个亚组。检测各组大鼠体质量、HE染色检测肺组织病变、实时定量PCR检测肺组织肿瘤坏死因子α(TNF-α)mRNA、ELISA检测血浆IL-1β水平。结果高氧暴露1 d,三组大鼠体质量差异不显著,肺组织病理未见异常。高氧后3、7 d,高氧组体质量明显低于对照组和治疗组,血浆IL-1β水平显著增高,肺组织结构破坏,7 d损伤更显著;高氧组肺组织TNF-αmRNA水平增高,IL-22治疗后,肺组织TNF-αmRNA水平明显降低。结论 IL-22对高氧诱导的肺损伤有保护作用。Objective To study the protective effect of interleukin-22 （IL-22） against the hyperoxia-induced lung injury in neonatal SD rats. Methods The neonatal SD rats were randomized into control group, hyperoxia group and IL-22 （ 10 ng/g） treatment group. Each group was randomly divided into three subgroups of 1, 3, 7 days （n =9）. Body mass in every group was detected; lung pathological changes were observed by HE staining; tumor necrosis factor α （TNF-α） in lung tissues was tested by quantitative real-time PCR; and IL-1β in plasma was measured by ELISA. Results After exposure to hyperoxia for 1 day, the body mass in the three groups showed no significant difference, and the structure of lung tissues were normal. After exposure to hyperoxia for 3 or 7 days, the body mass in the hyperoxia group was significantly lower, IL-1β in plasma was significantly enhanced, and the structure of lung tissues were destroyed, which aggravated with the time of hyperoxia exposure. TNF-a mRNA level increased obviously in the hyperoxia group. However, when treated with IL-22, TNF-α mRNA was significantly down-regulated in the treatment group. Conclusion IL-22 may play an protective role in hyperoxia-induced lung injury.

关 键 词：高氧肺损伤 白细胞介素22(IL-22) 白细胞介素1β(IL-1β) 肿瘤坏死因子α(TNF-α) 

分 类 号：R563[医药卫生—呼吸系统] R852.15[医药卫生—内科学]