原发性胆汁性肝硬化患者血清和肝组织白细胞介素9表达增强  

Increased expression of IL-9 in peripheral blood and liver tissues of patients with primary biliary cirrhosis

作　　者：王倩[1] 罗德梅[1] 孙晓凤[1] 冯宁[1] 李慧君[1] 胡金伟[1] 杨文强[1,2] 马秀敏[1,2] 

机构地区：[1]新疆医科大学第一附属医院,新疆重大疾病医学重点实验室-省部共建国家重点实验室培育基地,新疆乌鲁木齐830011 [2]新疆医科大学基础医学院,新疆乌鲁木齐830011

出　　处：《细胞与分子免疫学杂志》2016年第12期1679-1682,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：新疆自治区自然基金(2013211211A094);新疆医科大学第一附属医院项目(2013ZRQN30);新疆高校科研计划(XJEDU2013I20)

摘　　要：目的探讨白细胞介素9(IL-9)在原发性胆汁性肝硬化(PBC)患者中的表达水平及其临床意义。方法选取80例PBC患者和100例健康人,采用流式微珠阵列(CBA)法检测血清IL-9、γ干扰素(IFN-γ)、IL-6、肿瘤坏死因子-α(TNF-α)、IL-10和IL-17水平,实时荧光定量PCR检测外周血单个核细胞(PBMC)IL-9 mRNA水平,免疫组织化学染色检测肝组织IL-9表达和定位。结果与健康人相比,PBC患者血清IL-9、TNF-α、IL-6、IL-17、IFN-γ、IL-10均显著升高,且IL-9与Ig G水平正相关。PBC患者外周血中IL-9 mRNA水平升高,PBC肝组织中有IL-9阳性细胞,主要位于汇管区的胆管上皮细胞。结论 PBC患者外周血和肝组织中高表达IL-9。Objective To investigate the expression and clinical significance of interleukin 9 （IL-9） in patients with primary biliary cirrhosis （PBC）. Methods The study enrolled 80 PBC patients and 100 healthy subjects. Levels of serum IL-9, interferon y （IFN-y）, IL-6, tumor necrosis factor α （TNF-α）, IL-10 and IL-17 were determined by cytometric beads array （CBA） ; level of IL-9 mRNA in peripheral blood mononuclear cells （PBMCs） was detected by quantitative real-time PCR; the expression and location of IL-9 in liver tissues were detected by immunohistochemistry. Results Serum levels of IL-9, TNF-α, IL-6, IL-17, IFN-γ and IL-10 significantly increased in PBC patients in comparison with healthy controls, and a positively correlation between IL-9 and IgG was observed in PBC patients. In addition, the expression of IL-9 mRNA in PBC groups increased. Immunohistochemical results suggested that positive cells appeared in the PBC liver tissue, mainly in the portal area. Conclusion Expression of IL-9 remarkably increases in peripheral blood and liver tissues in patients with PBC.

关 键 词：原发性胆汁性肝硬化 白细胞介素9(IL-9) 

分 类 号：R657.31[医药卫生—外科学] R392.11[医药卫生—临床医学]