沙漠干热环境中暑大鼠血常规及肠黏膜病理学变化  

Investigation of Blood Routines and Intestinal Mucosal Pathologies of Dry-heat Stroke Rats

作　　者：董翔[1,2] 王金泉[1] 刘江伟[2] 许琴[2] 张东辉[2] 李佳佳[2] 马娜[2] 许永华[2] 

机构地区：[1]新疆农业大学动物医学院,乌鲁木齐830052 [2]新疆军区总医院新疆特殊环境医学重点实验室,乌鲁木齐830000

出　　处：《实验动物科学》2016年第6期16-20,共5页Laboratory Animal Science

基　　金：新疆维吾尔自治区自然科学基金面上项目(No.2015211C241)

摘　　要：目的研究沙漠干热环境下不同程度中暑大鼠血常规及肠黏膜的病理学变化。方法将SPF级SD雄性大鼠48只,随机分为常温对照组(NC组)、轻度中暑组(LHS组)、中度中暑组(MHS组)、重度中暑组(SHS组)。置于"西北特殊环境人工实验舱"模拟的沙漠干热环境温度(41±0.5)℃,湿度(10±2)%RH,禁食禁水。分别于(50±5)min、(100±5)min、(150±5)min造成大鼠轻、中、重度中暑模型后,立即用3%戊巴比妥钠腹腔深度麻醉处死,留取静脉血及回、结肠组织标本,进行血常规检测和病理学观察评分。结果大鼠随中暑程度的加深,白细胞数逐渐增加,SHS组白细胞数较其它各组增加明显(P<0.05);红细胞数、红细胞压积MHS组较NC组、LHS组增加明显,SHS组较其它各组增加明显(P<0.05);MHS组血小板数较NC组、SHS组增加明显(P<0.05)。MHS组、SHS组回肠和结肠病理学损伤评分随中暑程度的加深显著增加(P<0.05);MHS组、SHS组内回肠病理学损伤评分高于结肠(P<0.05)。结论沙漠干热环境造成中暑大鼠白细胞数、红细胞数、红细胞压积升高,血小板数呈现先升高后降低的趋势,肠黏膜病理学变化随中暑程度的加深而进行性加重,其中回肠较结肠病理学损伤更为严重,提示预防沙漠干热环境中暑对小肠黏膜的保护尤为重要,而血小板数降低可能是反应重度中暑的重要指标之一。Objective To investigate the changes of blood routines and intestinal mucosal pathologies of different degree heat stroke rats in dry-heat environment of desert. Method Forty eight male SD rats, SFP grade, were randomly divided into normal control group, light heat stroke group, moderate heat stroke group and severe heat stroke groups. Placed in simulated dry-heat environment of desert in The Simulated Climate Cabin for Special Environment of Northwest of China, temperature of （41 ± 0.5）℃, humidity of （ 10 ± 2） % RH, fasting and water deprivation, for （50 ± 5） min, （ 100 ± 5） min, （ 150 ±5 ） min respectively to cause light, moderate, severe heat stroke rat models. Then the rats were deep anesthetized and sacrificed immediately with 3% sodium pentobarbital intraperitoneally, samples of venous blood and ileum and colon tissue were collected to examine blood routines and count pathological histology score. Result Rats with accumulated degree of heat stroke, white blood cell count increased gradually, white blood cell count of SHS group compared with the other groups increased significantly （P 〈0.05）; red blood cell count and hematocrit of MHS group compared with the NC group and LHS group increased significantly, SHS group increased significantly, too （P 〈 0. 05） ; platelet count of MHS group compared with the NC group and SHS group increased significantly （P 〈 0.05）. The pathology injury score of ileum and colon were significantly increased with increasing heat degree in MHS group and SHS group （P 〈 0.05）. The ileum pathology injury score of MHS and SHS groups were higher than that of colon （P 〈 0.05）. Conclusion White blood cell count, red blood cell count, hematocrit of heat stroke rats, in dry-heat environment of desert, were increased, platelet count showed a trend of firstly increased and then decreased, intestinal mucosa pathological changes with increasing heat degree aggravated. Ileum pathology injury compared with colon is more serious. Our results s

关 键 词：干热环境 大鼠 肠黏膜 中暑 病理学 

分 类 号：Q494[生物学—生理学]