塞来昔布对海人藻酸致痫大鼠Iba-1及GFAP表达的影响  

Effect of Celecoxib on the Expression of Iba-1 and GFAP in Epileptic Rats Induced by Kainic Acid

作　　者：秦文艳[1] 齐越[1] 韦丹[2] 张冰冰[2] 康凯[2] 贾冬[2] 

机构地区：[1]辽宁省中医药研究院,沈阳110034 [2]辽宁中医药大学,沈阳110032

出　　处：《实验动物科学》2016年第6期31-34,共4页Laboratory Animal Science

基　　金：国家科技部"十二五""重大新药创制"专项课题(No.2012ZX09102201-005)

摘　　要：目的研究选择性COX-2抑制剂塞来昔布对癫痫大鼠的保护作用。方法采用海马内注射海人藻酸(Kainic Acid,KA,1μg/μL,1.0μL)建立大鼠癫痫模型,观察并记录各组大鼠注射KA后的行为学变化;采用免疫组化法检测大鼠海马组织中离子钙接头蛋白分子(Iba-1)和胶质纤维酸性蛋白(GFAP)的表达情况。结果塞来昔布组的癫痫发作潜伏期明显延长,最大发作级别明显降低(P<0.05);与模型组相比,塞来昔布组的海马组织中Iba-1和GFAP的表达减少,胶质细胞活化情况明显减轻。结论塞来昔布可能通过抑制小胶质细胞和星形胶质细胞活化,减轻其介导的炎症反应,改善癫痫发作的程度。Objective To study the protective effct of celecoxib on epileptic rats. Method Kainic acid（KA,1μg/μL,1.0μL）was injected into hippocampus to establish epilepsy model in rats and surveyed the behavioristics changes; immunohistochemical analysis was used to detect the Iba-1 and GFAP of hippoeampus. Result Compared with model group, celecoxib group extended the incubation and reduced the largest seizures rank significantly; decreased the expression of Iba-1 and GFAP, attenuated the hippocampus gllal activation caused by KA. Conclusion Celecoxib may inhibit the activity of gia cell to alleviate the gial-mediated inflammation, and lighten the degree of epilepsy.

关 键 词：塞来昔布 癫痫 Iba-1 GFAP 炎症 

分 类 号：R96[医药卫生—药理学]