预做脐带血保存的孕产妇标本核酸检测结果分析  

Nucleic acid testing result analysis of pre-made cord blood preserved in maternal specimens

作　　者：李玲[1] 欧国进[1] 李文慧[1] 陈阳 王珏[1] 谯丹 张莹[1] 刘晓[1] 王向明[1] 洪树青[3] 陈骁 陈强 刘忠[1] 

机构地区：[1]中国医学科学院北京协和医学院输血研究所,四川成都610052 [2]四川省新生命干细胞库 [3]安徽医科大学公共卫生学院

出　　处：《中国输血杂志》2016年第12期1343-1345,共3页Chinese Journal of Blood Transfusion

基　　金：科技部科研院所技术开发项目(2014EG150133);协和青年基金(3332015121)

摘　　要：目的了解四川省预做脐带血保存的孕产妇标本核酸检测情况,评价对预做脐带血保存的孕产妇标本进行核酸的意义。方法于2015年7-12月,收集预在四川省脐带血造血干细胞库做脐带血保存的孕产妇标本2管,先用酶联免疫法(ELISA)进行筛查,如果ELISA结果阳性,不再进行核酸检测;如果ELISA结果是阴性,则将其另一管标本送到输血研究所进行核酸检测。结果共收集到ELISA检测结果阴性标本4 878例,经核酸检测,共鉴别出HBV DNA阳性标本7例,无HIV RNA阳性和HCV RNA阳性结果。结论对于ELISA检测阴性的标本有必要进行核酸检测,对指导脐带血能否保存和避免输注脐带血传播病原体具有重要意义。Objective To understand the nucleic acid testing （NAT） results of pre-made cord blood preserved in ma- ternal specimens in Sichuan and to evaluate the significance of NAT. Methods All negative samples of ELISA screening were collected in Sichuan New Life Stem Cell Bank and sent to IBT between Jul, 2015 and December, 2015. Results There were 4 878 samples that were ELISA negative and were collected. Of these, 7 HBVDNA reactive samples were identified by NAT, and there was no HCVRNA and HIVRNA reactive results. Conclusion It is necessary that the ELISA negative speci- mens be tested by nucleic acid test. NAT has great significance in guiding cord blood preserved in maternal specimens and a- voiding spreading of transfusion transmitted pathogens.

关 键 词：脐带血 HBV ELISA 核酸检测 

分 类 号：R446[医药卫生—诊断学] R457.1[医药卫生—临床医学]