镇肝熄风汤对帕金森病肝阳上亢证大鼠旋转行为的影响  

Effects of Zhen Gan Xi Feng (ZGXF) Decoction on Amphetamine-Induced Rotation in Rats with Parkinson's Disease (PD) with the Syndrome of Liver-Yang Hyperactivity

作　　者：李晓明[1] 张丽华[2] 綦艳秋[2] 孙影[2] 朱兰芹[2] 董妙先[2] Li Xiaoming Zhang Lihua Qi Yanqiu Sun Ying Zhu Lanqin Dong Miaoxian(The Institute of Medicine, Qiqihar Medical Univeristy, Qiqihar 161006, China Health Center, Qiqihar Medical Univeristy, Qiqihar 161006, China)

机构地区：[1]齐齐哈尔医学院医药科学研究所,齐齐哈尔161006 [2]齐齐哈尔医学院卫生所,齐齐哈尔161006

出　　处：《世界科学技术-中医药现代化》2016年第12期2137-2142,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委面上项目(81373629):基于PI3K/Akt/GSK-3β信号通路的镇肝熄风法抑制帕金森病氧化应激损伤的机制研究;负责人:董妙先

摘　　要：目的:观察镇肝熄风汤对帕金森病(Parkinson’s Disease,PD)肝阳上亢证模型大鼠旋转行为的影响,探讨其可能的作用机制。方法:采用附子汤灌胃联合6-OHDA偏侧毁损制备PD肝阳上亢证大鼠模型,肝熄风汤灌胃给药28天。观察阿朴吗啡诱导的大鼠旋转行为,采用实时荧光定量PCR检测编码核因子E2相关因子2(Transcription Factor NF-E2-Related Factor 2,Nrf2)基因Nfe2l2和编码血红素加氧酶-1(Heme Oxygenase 1,HO-1)的基因Hmox-1的表达,采用Western blot检测Keap1蛋白表达。结果:与模型组比较,镇肝熄风汤显著减少了阿朴吗啡诱导的PD大鼠旋转行为,上调了模型大鼠中脑黑质Nfe2l2和Hmox-1 m RNA表达,抑制了Keap1蛋白表达。与假手术组比较,模型组大鼠中脑黑质Nfe2l2和Hmox-1 m RNA的代偿性增加也达到了统计学意义,但Keap1蛋白表达无显著性改变。结论:镇肝熄风汤可改善PD肝阳上亢证大鼠的行为学改变,增加中脑黑质Nfe2l2和Hmox-1 m RNA表达,抑制了Keap1蛋白表达,提示镇肝熄风汤抗PD的作用可能与其抗氧化作用有关。The aim of this study was to explore the effects of ZGXF decoction on amphetamine-induced rotation in PD rats with syndrome of liver-yang hyperactivity, and its mechanism involved. Rats received 6-OHDA administration via intra-substantia nigra injection and were intragastrically treated by Fu Zi decoction to establish the PD model with the syndrome of liver-yang hyperactivity. Three doses of ZGXF decoction or selegiline were given by a 28-day intragastric administration. The rats were tested for amphetamine-induced rotation asymmetry. In addition, real-time PCR were adopted to analyze the expressions of Nfe2l2 and Hmox-1 m RNAs, while western blot to analyze the expression of Keap1 protein. As a result, it was found that ZGXF decoction dose-dependently attenuated amphetamine-induced rotation, up-regulated the expressions of Nfe2l2 and Hmox-1 m RNAs, and down-regulated the expression of Keap1 protein in the substantia nigra in PD rats with syndrome of liver-yang hyperactivity. It was suggested that anti-PD effects of ZGXF decoction be attributed to the up-regulation of Nfe2l2 and Hmox-1 m RNAs and the down-regulation of Keap1 protein, being associated with oxidative stress, in the substantia nigra of PD rats with syndrome of liver- yang hyperactivity.

关 键 词：镇肝熄风汤 旋转行为 核因子E2相关因子2 肝阳上亢证 帕金森病 

分 类 号：R2-031[医药卫生—中西医结合]