双同位素示踪定量微藻对碳源利用份额的方法研究  

Application ofbidirectional labeling method to quantifying carbon utilization in microalgae

作　　者：李海涛[1,2] 吴沿友[2] 赵丽华[2] 张开艳[2] 杭红涛[1,2] LI Haitao WU Yanyou ZHAO Lihua ZHANG Kaiyan HANG Hongtao(School of Karst Science, Guizhou Normal University, Guiyang,Guizhou 550001, China State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang,Guizhou 550002, China)

机构地区：[1]贵州师范大学喀斯特研究院,贵阳550001 [2]中国科学院地球化学研究所环境地球化学国家重点实验室,贵阳550002

出　　处：《中国岩溶》2016年第6期614-618,共5页Carsologica Sinica

基　　金：国家自然科学基金项目(40973060);贵州省科学技术基金(黔科合J字[2014]2131号)

摘　　要：2015年8月,在人工温室培养环境下,以蛋白核小球藻为实验材料,通过向培养液中添加两种标记δ^(13)C的碳酸氢钠来培养微藻,每天定时监测培养液的无机碳稳定同位素组成和微藻生物量,并测定最终获得的微藻藻体的有机碳稳定同位素组成,运用双同位素示踪模型,通过无机碳稳定同位素和有机碳稳定同位素两种方法,分别成功计算出了微藻利用不同碳源的份额,实验结果分别为:添加5.0mmol/L碳酸氢钠条件下是0.19、添加10.0mmol/L条件下是0.37、而添加20.0 mmol/L条件下是0.57。并对这两种算法进行了分析。定量计算微藻对不同无机碳源的利用份额,在岩溶湖泊碳循环研究领域具有重要意义。August 2015, the Chlorella pyrenoidosawas cultivated in a greenhouse with different inorganic car- bons（NaH13CO3with different δ13C values added） in the culture medium. The δ13C of inorganic carbon in the medium and the biornass of the microalgae were detected on a daily basis. In the meantime, the organic sta- ble carbon isotope compositions of the microalgae was also measured. The proportion of the added inorganic carbon used by rnicroalgae was quantified by comparing their stable carbon isotope compositions using the bi- directional labeling method （NaH13CO3with different 813 Cvalues was added）. This study compared respec- tively both inorganic carbon and organic carbon of the stable carbon isotope compositions. The results are as follows, it is 0.19 under 5.0 mmol/L NaHCO3, 0.37 under 10.0 mmol/L NaHCO3, and 0.57 under 20.0 mmol/L NaHCO3. At last, we analyzed the two methods for quantifying the carbon sources in algae. It is very important to calculate the proportion of different inorganic carbon in the carbon cycle research in karst lakes.

关 键 词：碳酸盐利用能力 稳定碳同位素 微藻 

分 类 号：Q945[生物学—植物学] P642.25[天文地球—工程地质学]