前列腺素F_(2α)受体激动剂fluprostenol对奶牛输卵管上皮细胞合成分泌细胞因子表达的影响  

Effect of F_(2α) receptor agonist fluprostenol on mRNA expression of TGF-α,TGF-β3,FGF-2,LIF and GM-CSF in bovine oviduct epithelial cell

作　　者：张莹[1] 曹金山[1] 张楠[1] 

机构地区：[1]内蒙古农业大学兽医学院,内蒙古呼和浩特010010

出　　处：《中国兽医学报》2017年第2期345-351,共7页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31160490)

摘　　要：为验证前列腺素类化合物对输卵管分泌细胞因子有调控作用,以前列腺素F2α受体激动剂fluprostenol对奶牛输卵管上皮细胞合成分泌细胞因子TGF-α、TGF-β3、FGF-2、LIF和GM-CSF的调控机制为研究对象,采用实时荧光定量PCR技术检测吲哚美辛(10-5 mol/L)作用不同时间(2,4,8,16,24,48h)对奶牛输卵管上皮细胞TGF-α、TGF-β3、FGF-2、LIF和GM-CSF mRNA表达的影响。结果显示,fluprostenol对奶牛输卵管上皮细胞中TGF-α和FGF-2mRNA有正调控的作用,均在4h时mRNA的表达量达到峰值;fluprostenol对奶牛输卵管上皮细胞中LIF和GMCSF mRNA有双重调控作用,均是先短暂显著促进(P<0.05)其mRNA的表达,之后表现为极显著抑制(P<0.01),并均在2h时达到表达量峰值;fluprostenol对奶牛输卵管上皮细胞中TGF-β3mRNA起到正调控作用,作用48h时与空白对照组相比极显著升高(P<0.01),达到峰值。To reveal the regulation of the prostaglandin F2α receptor agonist fluprostenol on mRNA expression of TGF-α, TGF-β3, FGF-2, LIF and GM-CSF in bovine oviduct epithelial cell. Real time PCR were used to detect TGF-α, TGF-β3, FGF-2, LIF and GM-CSF mRNA expression in bovine o viduet epithelial cells after （fluprostenol, indomethacin （10-5mol/L） ） treatment at indicated time points （2,4,8,16,24,48 h）. Fluprostenol promoted the mRNA expression levels of TGF -α and FGF-2 in bovine oviductal epithelial cells,and both reached the maximum at 4 h. Fluprostenol had dual regulation to LIF and GM-CSF,and both reached the maximum at 2 h significantly incleased compared with blank group （P〈0. 05） ,and then,the expression of these cytokine was extremely decreased （P〈0.01）. Fluprostenol promoted the mRNA expression levels of TGF-β3 ,reached the maximum at 48 h,significantly increased （P〈0. 01） ,compared with blank group.

关 键 词：前列腺素F2α受体激动剂 fluprostenol 奶牛输卵管上皮细胞 TGF—α TGF-β3 FGF2 LIF GM—CSF 

分 类 号：S814[农业科学—畜牧学]