油茶叶肿病变态叶片的形态特征和超微结构观察  

Observation on Morphological Traits and Ultrastructure of Abnormal Leaf Induced by Exobasidium gracile in Camellia oleifera

作　　者：黄瑞君[1] 王兆强[1] 杨阳[1] 李竞[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨陵712100

出　　处：《西北植物学报》2017年第1期8-13,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：西北农林科技大学引进人才科研启动经费

摘　　要：利用扫描电镜(SEM)对油茶叶肿病变态叶叶片表面和横切面进行观察,利用透射电镜(TEM)对其细胞超微结构进行观察,以期探明油茶叶肿病变态叶的形态特征和细胞学特征。结果表明:(1)变态叶是受感染油茶幼叶组织增生形成的,肿大的叶片厚度比正常叶片厚度增加3~5倍,细胞体积增大3~8倍,细胞数增加1~2倍,叶片细胞形态和结构发生了变化。(2)叶片受细丽外担菌侵染后,菌丝存在于下表皮向内的4~7层细胞间隙中,感染后期叶片下表面脱落露出子实层。(3)变态叶细胞出现叶绿体膜破裂、类囊体片层膜数目减少及细胞器成分被破坏等异常现象。The morphological traits and ultrastructure of Camellia oleifera leaf were observed, using resin embedding technology, scanning electron microscopy （SEM） and transmission electron microscopy （TEM） methods. The results indicated that： （1） The abnormal leaf was proliferative tissues of infected young leaf, compared with normal leaves. The thickness of the swelling leaf increased 3 to 5 times, the cell volume increased 3 to 8 times, and the number of cells increased 1 to 2 times. （2） Hyphae exist in the lower epidermis to layer 4-7 in the intercellular then epidermis exfoliated from the lower surface of the leaf at later stages. （3） Several abnormal phenomenons such as the rupture of chloroplast membranes, the decrease of the numbers of internal thylakoid membranes of chloroplast and the damaged structure of organelles were observed.

关 键 词：油茶 细丽外担菌 形态特征 超微结构 

分 类 号：Q944.56[生物学—植物学]