^(60)Co-γ射线辐射谷子干种子诱变效应的研究  

Mutagenic Effects of ^(60)Co-γ-Ray Radiation on Dry Seeds of Foxtail Millet

作　　者：赵丽娟[1,2] 马金丰[2] 李延东[2] 李祥羽[2] 李志江[2] 袁红梅[3] 郭文栋[4] 

机构地区：[1]黑龙江省农业科学院博士后科研工作站,黑龙江哈尔滨150086 [2]黑龙江省农业科学院作物育种研究所,黑龙江哈尔滨150086 [3]黑龙江省农业科学院经济作物研究所,黑龙江哈尔滨150086 [4]黑龙江省科学院自然与生态研究所,黑龙江哈尔滨150040

出　　处：《作物杂志》2017年第1期38-43,共6页Crops

基　　金：黑龙江省自然科学基金面上项目(C201316);国家谷子糜子产业技术体系(CARS-07-13.5-B15);哈尔滨市科技局青年后备人才项目(2014RFQYJ138)

摘　　要：为了研究γ射线在谷子诱变育种中的应用,本研究利用4个不同剂量的^(60)Co-γ射线辐照4份不同熟期的谷子材料。取少量辐射后的种子做发芽率分析,并对M_1代的出苗期、出苗率、成株率、畸变苗率等进行统计,在M_2代筛选突变株。结果表明,辐射剂量在0.20~0.35KGY范围内,对发芽率没有显著影响;0.20KGY的剂量能促进谷子出苗,随着辐射剂量加大转为抑制出苗;随着辐射剂量的增加,出苗日期延迟,成株率呈下降趋势,畸变苗率呈上升趋势;4个供试材料对辐射的敏感性有明显的差异,各品种(系)适宜的辐射剂量有所不同。辐射敏感性由强到弱依次是:朝谷3-1>龙选1>龙谷32>龙谷25;各品种(系)适宜的辐射剂量是:朝谷3-1为0.25KGY,龙谷32为0.25KGY,龙谷25为0.30KGY,龙选1为0.30KGY。并对突变规律进行初步探索。In order to study the application of γ-ray in the mutation breeding of foxtail millet, four different doses of 60Co-γ ray were used to irradiate four different foxtail millet cultivars. A small amount of seeds after radiation were used for germination rate analysis. Seedling emergence date, seedling emergence rate, surviving plant rate, seedling ditstortion rate, etc of M1 generation were recorded, and the mutant individuals were screened in M2 generation. The results showed that there was no significant effect on germination rate in the dosage range of 0.20-0.35KGY, but the delayed emergence date extended, surviving plant rate decreased and seedling ditstortion rate increased with the increase of the dosage of irridiation. Radio sensitivity and suitable dosage of the four tested cultivars was significantly different. Radio sensitivity from strong to weak was： Chaogn 3-1, Longxuan 1, Longgu 32, Longgu 25. Suitable dosage of the varieties： Chaogu 3-1 for 0.25KGY, Longgu 32 for 0.25KGY, Longgu 25 for 0.30KGY, Longxuan 1 for 0.30KGY.

关 键 词：谷子 60Co-γ射线 辐射敏感性 适宜辐射剂量 

分 类 号：S515[农业科学—作物学]