DNA条形码在主要滇产石斛鉴别中的应用  

Application of DNA Barcoding in species identification of Dendrobium widely used in Yunnan

作　　者：张爱丽[1] 高亚芳[1] 黎太米 谭文红[1] 钱子刚[1] ZHANG Aili GAO Yafang LI Taimi TAN Wenhong QING Zigang(Yunnan University of Chinese Traditional Medicine, Kunming 650500, Chin)

机构地区：[1]云南中医学院,云南昆明650500

出　　处：《中国民族民间医药》2017年第2期11-16,共6页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：基金项目:中药现代化科技产业基地建设(2014RE021)

摘　　要：目的:选择云南境内应用面较广、市场流通量较大的14个石斛品种,建立其DNA条形码鉴定方法。方法:结合Genbank数据库序列,选择ITS、psbA-trnH、matK、rbcL等4条常用序列对石斛DNA样品进行扩增和测序,利用MEGA5.0计算种间种内K2P遗传距离并构建NJ树,同时采用Taxon DNA软件计算Barcoding gap(条形码种内种间遗传差异)。结果:ITS序列的K2P种内遗传距离小于种间遗传距离,NJ树可以很好地将不同品种的石斛分开,且有较明显的Barcoding gap。结论:ITS序列可以作为鉴别这14种滇产主要石斛的优选序列。Objective To identify 14 species of Dendrobium which widely used in Yunnan province by DNA barcoding.Methods Three chloroplast sequences including psbA-trnH,matK,rbcL and an ribosomal DNA ITS sequence of the 14 species were compara-tively analyzed.K2P distances were calculated and NJ Tree was performed applying MEGA 5. 0. Meanwhile,Barcoding gap were calcu-lated by Taxon DNA.Results The interspecific K2P distance of ITS were higher than the intraspecific one.And NJ tree could also di-vide species into different clades.The barcoding-gap were obvious between intra-and inter-species.Conclusions ITS sequence can be the optimal barcode to identify the 14 species of Dendrobium.

关 键 词：滇产石斛 DNA条形码 分子鉴别 

分 类 号：R197[医药卫生—卫生事业管理]