番茄叶醇提物急性毒性及其抗氧化应激损伤研究  

Acute Toxicity and Protective Effects of Tomato Leaf Alcohol Extract against Oxidative Stress Injury Induced by Acute Inflammatory

作　　者：陆宏浩 卫智权[2] 阎莉[2] 覃世椿 LU Honghao WEI Zhiquan YAN Li Qin Shichun(Ruikang Affiliated Hospital of Guangxi Traditional Chinese Medical University, Nanning 530012, China Guangxi Traditional Chinese Medical University, Nanning 53000, China)

机构地区：[1]广西中医药大学附属瑞康医院,广西南宁530012 [2]广西中医药大学,广西南宁530001

出　　处：《中国民族民间医药》2017年第2期52-53,56,共3页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：广西高等学校高水平创新团队及卓越学者计划资助项目(桂教人〔2014〕7号);广西高校重点实验室<壮医方药基础与应用研究重点实验室>(桂教科研[2014]6号)

摘　　要：目的:探讨番茄叶醇提物的急性毒性及其对脂多糖(LPS)诱导急性炎症致氧化应激损伤大鼠的保护作用。方法:急性毒性试验是采用经口途径给予番茄叶醇提物,测定小鼠的半数致死量(LD50),并观察急性中毒表现。50只SD大鼠分为正常组、模型组与番茄叶醇提物6.54g/kg、3.27g/kg和2.18g/kg给药组,灌胃给药10 d。腹腔注射LPS复制急性炎症模型,以酶联免疫吸附剂法(ELISA)测定血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSHpx)和超敏C反应蛋白(Hs-CRP)水平。结果:番茄叶醇提物单次灌胃给药的LD50为65.39g/kg。番茄叶醇提物可明显提高急性炎症大鼠血清SOD、GSHpx水平,并显著降低Hs-CRP水平。结论:番茄叶醇提物可拮抗LPS引起的急性炎症的氧化应激损伤。In this research,acute toxicity of tomato leaf alcohol extract and protective effects of TLAE against oxidative stress injury in-duced by acute inflammatory were discussed.The oral lethal dosage （LD50 ）value of TLAE in mice is65.39g/kg obtained by using single dose acute toxicity testing for pharmaceuticals.Fifty SD rats were randomly divided into control group,model group and three TLAE treated groups of different dose （6. 54,3. 27,2. 18g/kg）.Acute inflammation was induced byintraperitoneal injection oflipopolysaccharide （4mg/kg）.TLAEwere orallygiven once a day for 10 days.The levels of SOD,GSHpx in serum were measured by enzyme-linked immunosorbent assay （ELISA）.TLAE enhanced the levels of SOD,GSHpxand Lower thelevel of Hs-CRP in serum.These results suggested that the orally given TLAE can markedly restrainthe oxidative stress injury with the LPS-induced acute inflammatory.

关 键 词：番茄叶醇提物 急性毒性试验 脂多糖 氧化应激损伤 

分 类 号：R285.5[医药卫生—中药学]