聚甲基丙烯酸甲酯骨水泥对肺癌细胞毒性作用的体外实验  

In vitro toxic effect of polymethylmethacrylate bone cement on lung cancer cells

作　　者：潘元星 米川[1] 施学东[1] 王冰[1] 崔云鹏[1] 林云飞[1] 

机构地区：[1]北京大学第一医院骨科,北京市100083

出　　处：《中国组织工程研究》2017年第2期187-191,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:经皮椎体成形是治疗脊柱转移瘤的一种微创手术方法,但术中注射的聚甲基丙烯酸甲酯(polymethylmethacrylate,PMMA)骨水泥对肿瘤的治疗机制目前尚不明确。目的:分析PMMA骨水泥及其单体对肿瘤细胞的细胞毒作用。方法:将固化期、拉丝期PMMA骨水泥的浸提液及不同质量浓度单体的稀释液与人肺腺癌细胞spc-A1接触培养1,3 d后,采用细胞形态直接观察法和CCK-8比色法测定细胞吸光度(A)值,计算细胞相对增殖率(RGR),评价PMMA骨水泥及其单体对肿瘤细胞的毒性。结果与结论:11 d 1 g/L组、10 g/L组的A值明显小于阴性对照组(P<0.01);3 d 1 g/L组、10 g/L组及拉丝期浸提液A值明显小于阴性对照组(P<0.05);2结果显示培养1 d 1 g/L组、10 g/L组的A值明显小于阴性对照组(P<0.01),细胞相对增殖率为26%-29%,毒性评价为4级;3 d 1 g/L组、10 g/L组的A值明显小于阴性对照组,细胞相对增殖率12%-16%,毒性评价为4至5级;3 d 100 mg/L组、拉丝期PMMA浸提液组的A值明显小于阴性对照组,毒性评价为2级。3结果表明,拉丝期PMMA骨水泥浸提液及PMMA单体质量浓度为100 mg/L-10 g/L时对肿瘤细胞有明显的毒性,说明经皮椎体成形过程中拉丝期PMMA骨水泥注入椎体后其释放的单体可能对肿瘤细胞存在细胞毒作用。BACKGROUND： Percutaneous vertebroplasty is a minimally invasive treatment for spinal metastasis tumor, but the mechanism of the injected polymethylmethacrylate（PMMA） bone cement in the treatment of tumor is not fully understood. OBJECTIVE： To explore the cytotoxicity of PMMA bone cement and its monomers on tumor cells. METHODS： PMMA extracts in the wire drawing and curing periods and different mass concentrations of monomer dilutions were co-cultured with human lung cancer cells spc-A1. The cell morphology was observed using inverted microscope, the absorbance（A） values were detected by cell counting kit- 8 assay, the relative growth rate was calculated, and the toxicity of PMAA bone cement and its monomers was evaluated at 1 and 3 days of culture, respectively.RESULTS AND CONCLUSION： At 1 day of culture, the absorbance values in the 1 and 10 g/L groups were significantly lower than that in the negative control group（P〈0.01）. The absorbance values in the 1 g/L, 10 g/L, 100 mg/L and wire drawing extract groups were significantly lower than those in the negative control group at 3 days of culture（P〈0.05）. The relative growth rate in the 1 and 10 g/L groups was 26%-29%, and the level of toxicity was grade 4 after 1-day culture; at 3 days, the relative growth rate was decreased to 12%-16%, and the level of toxicity was grades 4-5. After 3-day culture, the level of toxicity reached to grade 2 in the 100 mg/L and wire drawing extract groups. These results indicate that PMMA bone cement in wiredrawing period and its monomers exert toxic effects on tumor cells.

关 键 词：聚甲基丙烯酸类 椎体成形术 肿瘤 组织工程 生物材料 骨生物材料 经皮椎体成形术 聚甲基丙烯酸甲酯 骨水泥 细胞毒 肺癌细胞 体外试验 脊柱转移瘤 

分 类 号：R318[医药卫生—生物医学工程]