多孔磷酸钙颗粒对维生素C的负载及其可控释放  

Controlled release of porous calcium phosphate nanoparticles loaded with vitamin C

作　　者：孔军[1] 祝永强[2] 

机构地区：[1]浙江省立同德医院,浙江省杭州市310012 [2]浙江省中医药研究院,浙江省杭州市310007

出　　处：《中国组织工程研究》2017年第2期273-279,共7页Chinese Journal of Tissue Engineering Research

摘　　要：背景:近来有报道指出,维生素C可诱导、加速骨髓间充质干细胞向成骨细胞分化,促进骨的修复和再生,但维生素C水溶液不稳定,因此在其使用时需要载体材料的保护。目的:将磷酸钙作为维生素C的载体,观察其对维生素C的包载及控释能力。方法:采用化学沉淀法制备负载维生素C的磷酸钙颗粒,使维生素C终浓度分别为0,0.1,2,4 mmol/L,检测颗粒载药量。将负载维生素C的磷酸钙颗粒置于模拟体液中,检测维生素C释放量;同时检测超声环境下的维生素C释放量。将磷酸钙颗粒、负载2 mmol/L维生素C的磷酸钙颗粒与MC3T3-E1细胞共培养,1,3,5,7 d后检测细胞增殖,1,5,10,15 d后检测细胞碱性磷酸酶活性。结果与结论:1负载0.1,2,4 mmol/L维生素C磷酸钙颗粒的载药量分别为(59.9±5.4)%、(87.2±1.2)%及(28.4±26.3)%;2在正常环境下,负载0.1,2 mmol/L维生素C的磷酸钙颗粒前期释放速率较慢,负载4 mmol/L维生素C的磷酸钙颗粒前期释放速率较快,3种样品都有持续释药的特点;3在超声环境下,负载2 mmol/L维生素C的磷酸钙颗粒存在一定的突释现象,第1次释放量为5%-15%,而随后的释放速度较缓慢;在75,105和150 W超声辅助条件下,分别持续释放了220,340,260 min;4负载2 mmol/L维生素C的磷酸钙颗粒对MC3T3-E1细胞的增殖无影响,但提高了细胞的碱性磷酸酶活性;5结果表明,磷酸钙可作为维生素C的载体。BACKGROUND： It is reported that vitamin C can induce bone marrow mesenchymal stem cells differentiating into osteoblasts, and promote bone repair and regeneration. However, vitamin C solution is unstable, so a carrier is necessary.OBJECTIVE： To observe the loading and controlled-release abilities of calcium phosphate used as the carrier of vitamin C. METHODS： Calcium phosphate particles loaded with vitamin C were fabricated using chemical precipitation method, and the final concentration of vitamin C was 0, 0.1, 2 and 4 mmol/L, respectively. The drug-loaded capacity was detected. The release of vitamin from calcium phosphate nanoparticles in the simulate body fluid and ultrasonic environment was respectively evaluated. MC3T3-E1 cells were co-cultured with calcium phosphate nanoparticles loaded with 2 mmol/L vitamin C, or calcium phosphate nanoparticles only. The cell proliferation was detected at 1, 3, 5 and 7 days of culture, and the alkaline phasphatase activity was detected at 1, 5, 10 and 15 days of culture. RESULTS AND CONCLUSION： The drug-loaded contents of calcium phosphate nanoparticles loading 0, 0.1, 2 and 4 mmol/L vitamin C were（59.9±5.4）%,（87.2±1.2）% and（28.4±26.3）%, respectively. Under normal environment, all samples could release vitamin C persistently, but the initial release speed of the particles carrying 0.1 and 2 mmol/L vitamin C was lower than that of particles carrying 4 mmd/L vitamin. Under ultrasonic environment, 2 mmol/L vitamin C-loaded calcium phosphate particles exhibited a quick release speed firstly that reached 5-15%, followed by a slow release speed. When ultrasonic powers kept at 75, 105 and 150 W, the release duration of vitamin C was 220, 340 and 260 minutes, respectively. MC3T3-E1 cell proliferation did not change after co-cultured with 2 mmol/L vitamin C-loaded calcium phosphate particles but the alkaline phosphatase activity was improved. These results suggest that calcium phosphate particles can be used as the carrier of vitamin C.

关 键 词：磷酸钙类 药物载体 抗坏血酸 组织工程 生物材料 缓释材料 磷酸钙 维生素C 超声化学法 可控释放 

分 类 号：R318[医药卫生—生物医学工程]