胶原蛋白-明胶复合支架材料修复周围神经缺损  

Collagen-gelatin scaffolds for the repair of peripheral nerve defects

作　　者：刘天丹[1] 张保朝[1] 郝明亮[2] 

机构地区：[1]南阳市中心医院,河南省南阳市473000 [2]郑州大学第一附属医院,河南省郑州市450000

出　　处：《中国组织工程研究》2017年第2期286-290,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:文献报道显示,胶原蛋白-明胶复合支架可促进周围神经缺损的早期恢复,但该结论尚未得到进一步证实。目的:观察胶原蛋白-明胶复合支架的细胞相容性及修复周围神经缺损的效果。方法:将胶原蛋白-明胶复合支架与SD大鼠骨髓间充质干细胞共培养5 d,观察两者复合形态。切除20只SD大鼠左侧30 mm的腓总神经,建立周围神经缺损模型。随机分组,实验组神经缺损处植入复合骨髓间充质干细胞的胶原蛋白-明胶复合支架,对照组植入自体神经,植入后16周,进行桥接体中段形态观察与电生理检测。结果与结论:1细胞与支架复合形态:骨髓间充质干细胞在支架上生长良好,与支架融合良好;2桥接体中段形态观察:两组有髓神经纤维密度比较差异无显著性意义(P>0.05),实验组有髓神经纤维直径、髓鞘厚度及神经组织百分比显著低于对照组(P<0.05);3电生理检测:两组运动神经传导速度、运动神经潜伏期、感觉神经传导速度、感觉神经波幅比较差异无显著性意义(P>0.05),实验组运动神经波幅、感觉神经潜伏期显著低于对照组(P<0.05);4结果表明:胶原蛋白-明胶复合支架具有良好的细胞相容性,可用于修复周围为神经缺损。BACKGROUND： Collagen-gelatin composite scaffolds have been reported to be able to promote the early recovery of peripheral nerve defects. However, this conclusion has not been further confirmed. OBJECTIVE： To investigate the biocompatibility of the collagen-gelatin scaffold and its treatment outcomes in the repair of peripheral nerve defects.METHODS： The collagen-gelatin scaffold was co-cultured with bone marrow mesenchymal stem cells（BMSCs） of Sprague-Dawley rats for 5 days, and then the cell growth was observed. Twenty Sprague-Dawley rats were enrolled, modeled into a left 30-mm peroneal nerve defect and randomized into experimental and control groups. The collagen-gelatin scaffold composited with BMSCs was implanted into the experimental group, and autograft nerve implanted into the control group. Morphology of the middle bridge was observed, and electrophysiology was conducted at 16 weeks after implantation. RESULTS AND CONCLUSION： BMSCs grew and adhered well onto the scaffold. The myelinated nerve fiber density did not significantly differ between groups（P〈0.05）. The myelinated nerve fiber diameter, myelin sheath thickness and percentage of nerve tissues in the experimental group were significantly lower than those in the control group（P〈0.05）. There were no significant differences in the conduction velocity, latency of motor nerves and the conduction velocity and amplitude of sensory nerves between groups（P〈0.05）. The amplitude of motor nerves and the latency of sensory nerves in the experimental group were significantly lower than those in the control group（P〈0.05）. To conclude, the collagen-gelatin scaffold holds a good cytocompatibity and is ideal for the repair of peripheral nerve defects.

关 键 词：胶原 明胶 周围神经 组织工程 生物材料 材料相容性 胶原蛋白 周围神经缺损 冷冻干燥技术 骨髓间充质干细胞 感觉神经 运动神经 

分 类 号：R318[医药卫生—生物医学工程]