益智仁盐炙前后分别组成缩泉丸的缩尿部位指纹图谱比较  

Comparison on Fingerprint Spectrum of Urine-reducing Part of Suoquan Wan Constituted with Non-processed or Salt-processed Alpiniae Oxyphyllae Fructus Fruits

作　　者：李梦琪[1] 吴珊珊[1] 龚晓猛 刘斯琪[1] 胡昌江[1] 

机构地区：[1]成都中医药大学药学院,中药材标准化教育部重点实验室,四川省中药资源系统研究与开发利用重点实验室--省部共建国家重点实验室培育基地,成都611137

出　　处：《中国实验方剂学杂志》2017年第4期16-19,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81473353)

摘　　要：目的:通过比较益智仁盐炙前后组成缩泉丸缩尿部位的指纹图谱,从物质基础上揭示益智仁盐炙后增强缩泉丸缩尿药效的原因。方法:采用HPLC,流动相乙腈-水梯度洗脱,检测波长230 nm。记录10批益智仁盐炙前后分别组成缩泉丸的石油醚部位HPLC图谱,建立相应指纹图谱,标定特征共有峰并比较其平均相对峰面积和平均相对保留时间。结果:益智仁盐炙后组成缩泉丸时,有11个共有峰平均相对面积明显增加,包括对缩尿药效贡献较大的1号,4号(teuhetenone A),5号(7-表-香科酮),7号色谱峰,增加率分别为123.08%,90.91%,53.33%,43.75%。结论:对缩尿作用贡献较大的4个成分含量的明显增加极有可能与缩泉丸缩尿作用的增强紧密相关,此外其他7个成分含量也有明显增加。Objective： A comparison was made between fingerprint spectrum of urine-reducing part of Suoquan Wan constituted with non-processed and salt-processed Alpiniae Oxyphyllae Fructus fruits to discuss the chemical composition changes after salt-processing that enhance the urine-reducing effect. Method： HPLC was used for analysis with mobile phase of acetonitrile-water for gradient elution and detection wavelength at 230 nm.HPLC spectra of 10 batches Suoquan Wan constituted with non-processed and salt-processed Alpiniae Oxyphyllae Fructus fruits were recorded. Establishing the fingerprint spectra,32 peaks marked on each fingerprint spectrum had been compared. Result： The average relative area of 11 common peaks increased after salt-processing of Alpiniae Oxyphyllae Fructus fruits. Chromatographic peaks of No. 1,No. 4（ teuhetenone A）,No. 5（ 7-epiteucrenone B） and No. 7 were the major contribution to urine-reducing effect, which increased 123. 08%,90. 91%,53. 33% and 43. 75%,respectively. Conclusion： The change of those 4 components maybe closely related to the enhancement of urine-reducing effect of Suoquan Wan,and the change of other 7 components may be synergistic to it.

关 键 词：缩泉丸 益智仁 盐炙 指纹图谱 缩尿部位 圆柚酮 

分 类 号：R283.1[医药卫生—中药学] R284.1[医药卫生—中医学]