黄泥煨制人参中皂苷成分的RRLC-Q-TOF MS分析  

Study on Ginsenosides in Mud-Covered Processed-Ginseng by RRLC-Q-TOF MS/MS

作　　者：戴雨霖 庞博[2] 阎琪[1] 乔梦丹 郑飞[1] 越皓[1] 刘淑莹[1] DAI Yu-lin PANG Bo YAN Qi QIAO Meng-dan ZHENG Fei YUE Hao LIU Shu-ying(Changchun University of Chinese Medicine, Changchun 130117, China First Hospital of J ilin University, Changchun 130023, China)

机构地区：[1]长春中医药大学,吉林长春130117 [2]吉林大学第一医院,吉林长春130023

出　　处：《质谱学报》2017年第1期60-66,共7页Journal of Chinese Mass Spectrometry Society

基　　金：国家自然科学基金项目(31400682);吉林省科技发展计划项目(20160101152JC)资助

摘　　要：人参作为新资源食品,有多种炮制方法的应用。本研究对于黄泥煨制的人参化学成分是否安全、可靠提供了借鉴。利用高分离度快速液相色谱-四极杆-飞行时间质谱(RRLC-Q-TOF MS)法研究黄泥煨炮制方法的人参化学成分。鲜人参黄泥煨制的样品溶解离心过滤后,采用Agilent ZORBAX SB-C18色谱柱,以0.1%甲酸水-乙腈溶液作为流动相进行梯度洗脱,高分辨质谱进行检测,Masshunter Qualitative Analysis软件与人工相结合进行数据分析。结果表明,本研究鉴定了31种人参皂苷,比较了黄泥煨制人参与其他炮制方法人参中人参皂苷的相对百分含量差异,同时检测到F_2、Rg_3等稀有人参皂苷。该研究可为人参的使用提供更多的途径,能够为开发人参的多种保健功能提供依据。Panax ginseng C.A.Mey（ginseng）is a traditional Chinese medicine herb,which has been widely used for the treatment of enhancing intelligence and improving immunity in China and Aisa.As a new resource food in China,ginseng has a variety of processing methods which are used for different symptoms.The major active components of ginseng are ginsenosides,which demonstrate the ability to target a number of tissues and produce an array of pharmacological responses.In this study,active components from mud-covered processed-ginseng were studied whether the chemical composition of ginseng is safety and reliable.Rapid resolution liquid chromatography coupledwith quadrupole-time-of-flight tandem mass spectrometry（RRLC-Q-TOF MS/MS）was used to compare the white ginseng,vinegar processed ginseng and mud-covered processed ginseng for the composition of ginsenosides.Chromatographic conditions were as follows：ZORBAX SB-C18column（2.1mm×100mm×3.5μm）,column temperature of 35 ℃,mobile phase of water-acetonitrile gradient elution,flow rate of 0.3mL/min,injection volume of 5μL.Thirty-one ginsenosides were identified by the comparison of the retention times of the standard compounds and the accurate mass obtained from RRLC-Q-TOF MS/MS.A few minor ginsenosides,such as ginsenoside F2 and Rg3,were detected by the comparison of white ginseng with processed-ginseng.The method is applicable to the research of compounds in the processed Chinese herbs.

关 键 词：黄泥煨制 人参皂苷 液相色谱-质谱 

分 类 号：O657.63[理学—分析化学]