紫外分光光度法测定小儿清热宁颗粒中黄芩苷的含量  

Determination of Baicalin in Xiaoer Qingrening Granula by UV-Spectrophotometry

作　　者：郧海丽[1] 王美娟[1] 周冉[1] 雷霓[1] 申晓雷 YUN Hai-li WANG Mei-juan ZHO U Ran LEI Ni SHEN Xiao-lei(Department of Chemical Engineering, Shijiazhuang University, Shijiazhuang 050035, Chin)

机构地区：[1]石家庄学院化工学院,河北石家庄050035

出　　处：《化学试剂》2017年第2期172-174,共3页Chemical Reagents

基　　金：石家庄学院校级科研平台资助项目(XJPT008)

摘　　要：建立了一种用紫外分光光度法测定小儿清热宁颗粒中黄芩苷含量的方法。以90%乙醇溶液为空白溶剂,采用紫外分光光度法在276 nm下测定。黄芩苷乙醇溶液在3.04~9.12 mg/L浓度范围内与吸光度呈良好的线性关系,相关系数为0.999 8,平均回收率为99.9%,相对标准偏差为0.70%(n=9)。该方法简便快捷、数据准确可靠。The method of determination of baicalin in xiaoer qingrening granula by UV-spectrophotometry was established. The content of baicalin was determined by UV-spectrophotometry at 276 nm with the blank was 90% ethanol solution. The content of the baicalinethanol solution showed a better linear relationship with the absorbance in the range of 3. 04 - 9. 12 mg/L,correlation was 0. 999 8. The average recovery was 99. 9%,RSD was 0. 70%（n = 9）. The method was easy-to-handle with good accuracy and reproducibility.

关 键 词：紫外分光光度法 小儿清热宁颗粒 黄芩苷 

分 类 号：O657[理学—分析化学]