砂仁及其近缘药用植物的高效薄层色谱指纹图谱研究  

High-Performance Thin-Layer Chromatographic Fingerprint Analysis of Amomum villousm and Its Related Species

作　　者：邵艳华[1] 刘美廷[1] 杨一帆[1] 于现阔 丁平[1] SHAO Yan-hua LIU Mei-ting YANG Yi-fan YU Xian-kuo DING Ping(College of Chinese Materia Medica, Guang- zhou University of Chinese Medicine, Guangzhou 510006, China)

机构地区：[1]广州中医药大学中药学院,广州510006

出　　处：《中国药学杂志》2017年第3期188-192,共5页Chinese Pharmaceutical Journal

基　　金："十二五"国家科技支撑计划子课题(2011BAI01B01)

摘　　要：目的对砂仁及其近缘药用植物的挥发油成分进行高效薄层色谱指纹图谱研究,以期解决砂仁的专属性鉴别,并为其质量控制提供依据。方法采用高效薄层色谱法,控制相对湿度为67%,环己烷-三氯甲烷-乙酸乙酯(13∶2∶2)为展开剂,5%香草醛硫酸溶液显色,于可见光下检视,薄层色谱图用Chromap 1.5色谱软件进行分析。结果建立了由9个特征斑点构成的砂仁挥发油类成分的高效薄层色谱指纹图谱,并指认出主要成分乙酸龙脑酯。通过HPTLC指纹图谱考察,不同存放时间及不同产地的砂仁药材质量参差不齐;常见砂仁混伪品间质量差别显著;砂仁及姜科其他近缘种常用药用植物挥发油类成分的化学组成亦差异明显。结论该方法可以有效地鉴别砂仁,并评价其质量。OBJECTIVE To study the high-performance thin layer chromatographic （HPTLC） fingerprint of volatile oil constitu- ents from Amomum villousm and its related species so as to set up the identification protocol of the medicinal plant and provide scientific information for its quality control. METHODS TLC was used to analyze comparatively 10 batches of Amomum villosum Lour. samples, 10 batches of Amomum villousm crude drugs collected from different producing areas and stored for different time, 10 batches of the fruits of counterfeit species and 10 kinds of related species in the Zingiberaceae family. The samples were separated on silica gel G pre- coated plates with a mixture of cyclohexane-chloroform-ethyl acetate （13： 2： 2） as developing solvent system. The relative humidity was 67%. The spots were visualized with 5% vanillin sulfuric acid solution, then were analyzed by utilizing CHROMAP 1.5 solution soft- ware. RESULTS The fingerprint of volatile oil of Amomum villosum , with 9 specific bands examined under natural light, was set up. The quality of Amomum villosum stored for different time or collected from different areas was distinctly variable. Obvious difference ex- isted in the chemical composition of the volatile oils between Amomum villosum and its counterfeit and other related species. CON- CLUSION The HPTLC fingerprint analysis method can be used for rapid identification and quality control of Amomum villosum.

关 键 词：砂仁 混伪品 近缘药用植物 挥发油 高效薄层色谱法 

分 类 号：R284[医药卫生—中药学]