硫酸吲哚酚对人单核细胞源树突状细胞表型及功能的影响  

Indoxyl sulfate exerts an immunomodulatory effect on monocyte-derived-dendritic cells

作　　者：何栩[1] 吴奕章 傅强[1] 严全能[1] 梁欣伟[1] 李志樑[1] 

机构地区：[1]南方医科大学珠江医院心血管内科,广州市510280

出　　处：《实用医学杂志》2017年第2期194-197,共4页The Journal of Practical Medicine

基　　金：广东省省级科技计划项目(编号:2014A020212544)

摘　　要：目的:探讨硫酸吲哚酚(IS)对人单核细胞源树突状细胞(mDCs)的分化、成熟及免疫功能的影响,为进一步探索IS在动脉粥样硬化(AS)免疫炎症反应中的机制提供依据。方法:双密度梯度离心法分离人外周血单核细胞,用rh GM-CSF和rh IL-4体外诱导分化为未成熟mDCs,随机分为PBS组、LPS组(1μg/m L)、IS.1组(30μmol/L)、IS.2组(300μmol/L)、IS.3组(600μmol/L)。流式细胞仪检测各组mDCs表型和吞噬功能变化,ELISA法检测各组mDCs分泌细胞因子IL-12p70的变化,扫描电子显微镜观察细胞形态学变化。结果:不同浓度IS作用于mDCs后,显著上调mDCs表面标志CD80、CD83、CD86、MHC II的表达,降低细胞吞噬功能并促进细胞因子IL-12p70的分泌(P<0.05);形态学上看,LPS组和IS.2组细胞呈不规则形,胞体表面有形态不一的突起。比较得出IS为300μmol/L时为最适宜的刺激浓度。结论:硫酸吲哚酚可诱导人单核细胞源树突状细胞表型及功能成熟,这可能是硫酸吲哚酚参与动脉粥样硬化免疫炎症过程的机制之一。Objective To explore the effect of indoxyl sulfate（IS） on the differentiation, maturation andimmunological function of human monocyte derived dendritic cells（m DCs）, in order to provides evidence formechanism of IS in atherosclerosis. Methods Human peripheral blood mononuclear cells isolated by doublegradient centrifugation were cultured for immature m DCs by rh GM- CSF and rh IL- 4 in vitro. All cases wererandomly divided into PBS group, LPS group（1 μg/m L）, IS.1 group（30 μmol/L）, IS.2 group（300 μmol/L）andIS.3 group（600 μmol/L）. The phenotypic maturation of m DCs was evaluated by flow cytometry（FCM） andfunctional maturation of m DCs was analyzed by measuring FITC- dextran uptake and ELISA. Results ISsignificantly upregulated the expression of CD80, CD83, CD86 and MHC II key membrane molecules on m DCs,while downregulating phagocytosis and increasing the secretion of IL-12p70 by m DCs（P〈 0.05）. And the LPS andIS showed typical morphology with rough surface, long protrusions and fusiform. 300 μmol/L IS is the mostappropriate stimulus concentration. Conclusion Stuctural, phenotypic and functional maturation of dendritic cellsderived from human monocytes can be induced by indoxal sulphate at defined concentrations, which may be one ofthe mechanisms involved in the process of atherosclerosis.

关 键 词：动脉粥样硬化 树突状细胞 硫酸吲哚酚 免疫调节 

分 类 号：R54[医药卫生—心血管疾病]