化疗药物对CD19嵌合抗原受体T细胞的体外抑制作用  

The effect of chemotherapeutic drugs on CD19-CAR-T cells in vitro

作　　者：伊文芳[1] 杨默[1] 彭智勇[1] 何岳林[1] 刘华颖[1] 李春富[1] 

机构地区：[1]广东省珠海市人民医院儿科,519000

出　　处：《实用医学杂志》2017年第2期198-201,共4页The Journal of Practical Medicine

摘　　要：目的:利用CCK8法研究不同的化疗药物对嵌合抗原受体T(CAR-T)细胞的抑制作用,为临床制定化疗方案提供理论支持。方法:从正常成人外周血提取T细胞,制作成CD19-CAR-T细胞。将不同浓度化疗药物与CD19-CAR-T细胞共培养24,48,72 h和(或)96 h后,计算抑制率。结果:(1)氟达拉滨(Fludarabine,FDR)及马磷酰胺(Mafosfamide,MFA)对CD19-CAR-T细胞的抑制率随时间及浓度的增加而增加(P<0.05);(2)白舒非(Busulfan,BU)对CD19-CAR-T细胞在体外无抑制作用(P>0.05);(3)环磷酰胺(Cyclo-phosphamide,CTX)对CD19-CAR-T细胞在体外无抑制作用(P>0.05)。结论:在体外MFA及FDR对CD19-CAR-T细胞有抑制作用。CTX在体外无活性。Objective The research about the effect of different chemotherapeutic diugs on CD19-CAR-T cells with CCK8 test to provide the theoretical support about the development of chemotherapy for clinical support. Methods Extract T cells from a normal adult peripheral blood and synthesize CD19-CAR-T cell. CD19-CAR-T cells were treated with different doses of chemotherapeutic drugs for 24, 48, 72 h and （or） 96 h, and inhibition rate was calculated. Results First, we observed that the inhibition rates of fludarabine and Mafosfamide for CD19-CAR- T cells were increasing with the time and concentration （P 〈 0.05 ）. Secondly, Bus ulfan had no effect in CD19-CAR-T cells in vitro （P 〉 0.05）. Finally, Cyclophosphamide had no effect in CD19-CAR-T cells in vitro （P 〉 0.05 ）. Conclusion Mafosfamide and Fludarabine can inhibit the CD19-CAR-T cells. Cyclophosphamide have no activity in vitro.

关 键 词：CD19-CAR-T细胞 氟达拉滨 马磷酰胺 白舒非 环磷酰胺 

分 类 号：R96[医药卫生—药理学]