优化处理技术对男性不育症患者精子形态和DNA碎片指数的影响  

Effect of sperm spreparation in male infertility on sperm morphology and DNA integrity

作　　者：郑毅春[1] 徐丽清[1] 梁嘉颖[1] 唐婷[1] 汪李虎[1] 刘风华[1] 

机构地区：[1]广东省妇幼保健院生殖健康与不孕症科,广州市510010

出　　处：《实用医学杂志》2017年第2期231-234,共4页The Journal of Practical Medicine

基　　金：广东省中医药局科研课题资助项目(编号:20133001)

摘　　要：目的:确定优化处理技术对畸形精子症和不明原因不育症患者的精子形态和DNA碎片指数(DFI)的影响。方法:筛选2013年1月至2015年12月在我院就诊的400例男性及孕产妇家属,根据生育史和精液分析结果,分为正常生育组、畸形精子症组和不明原因不育症组,对其精液进行直接上游法和非连续密度梯度法优化处理,并分析其精子形态和DFI。结果:优化处理前畸形精子症组和不明原因不育症组精子头部、颈部畸形率和DFI均显著高于正常生育组(P<0.01);优化处理后正常生育组、畸形精子症组和不明原因不育症组的精子畸形率和DFI均显著低于处理前(P<0.01),经非连续密度梯度法处理的精子畸形率和DFI最低(P<0.01)。结论:相对于直接上游法,非连续密度梯度法更有利于筛选形态正常和DNA完整性高的精子。Objective To investigate the effect of sperm preparation in male infertility on sperm morphologyand DNA fragmentation index（DFI）. Methods Four hundred men were divided into the groups of fertile,teratozoospermics and unexplained subfertility. Sperm morphology and DFI were analyzed before and after thesperm preparation. Results Sperm abnormal morphology and DFI in the infertility group were higher than those inthe fertile group,but significantly decreased after sperm preparation. The method of density gradient separationresults in obtaining better sperm（P〈0.01）. Conclusions Compared to the method of swimming up,the methodof density gradient separation could result in obtaining sperm with improved normal morphology and DNA integrity.

关 键 词：男性不育症 精液优化处理 精子形态 精子DNA碎片指数 

分 类 号：R698.2[医药卫生—泌尿科学]