佐剂Al(OH)_3和CpG2216对福尔马林灭活呼吸道合胞病毒疫苗免疫增强的作用研究  

A study of enhancement immunopathogenesis effects of adjuvant Al(OH)_3 and CpG to respiratory syncytial virus vaccine

作　　者：李宏勇[1] 韩艳娟[1] 曾瑞红[2] LI Hong-yong HAN Yan-juan ZENG Rui-hong(Department of Pathogenic Biology and Immunology, Xing Tai Medical College, Xingtai 054000, Chin)

机构地区：[1]邢台医学高等专科学校,邢台054000 [2]河北医科大学免疫学教研室,石家庄050017

出　　处：《现代免疫学》2017年第1期50-54,共5页Current Immunology

基　　金：河北省自然科学基金(H2016206473)

摘　　要：研究佐剂Al(OH)_3与CpG2216对FI-RSV疫苗增强性免疫病理的不同作用,探寻FI-RSV疫苗增强性免疫病理的发生机制和解决方法,分别制备加入佐剂Al(OH)_3与CpG2216的FI-RSV疫苗,肌注免疫C57BL/6小鼠2次。免疫后观察小鼠发病情况,检测抗体水平、细胞因子转录表达并做肺组织切片等。实验发现两组血清IgG抗体产生无显著差异;FIRSV+CpG2216组小鼠有明显的发病迹象;病理切片显示,FI-RSV+Al(OH)_3组的黏液分泌最明显,FI-RSV+CpG2216组肺部显示更大量炎症细胞浸润,炎症反应较强;FI-RSV+CpG2216组肺组织RSV-N基因的表达量显著高于FI-RSV+Al(OH)_3组(P<0.05),FI-RSV+Al(OH)_3组的IFN-γ、IL-5、TNF-α、GATA-3、T-bet基因的表达量显著高于其他各组(P<0.05),FI-RSV+CpG2216组IL-17、ROR-γt基因的表达量显著高于其他各组(P<0.05)。认为CpG2216与Al(OH)_3单独作为佐剂在诱导抗体产生方面无明显差异;CpG2216单独作为佐剂引发了较强肺部炎症损伤和病理表现,可能因为其诱导了更强的Th17型优势应答所致;Al(OH)_3单独作为佐剂引发了较强的气管黏液分泌和Th2型优势应答。To compare the role and mechanism of Al（OH）3 and CpG as adjuvants to respiratory syncytial virus vaccine enhanced immu- nopathology, we prepared FI-RSV vaccine added Al（OH）3 or CpG respectiveIy, C57BL/6 mice were immunized by intramuscularly twice, and antibodies were detected. After RSV challenge, lung tissue sections were prepared and examined for the transcription of various cytokines. The level of IgG antibody in each immunized group showed no significant difference. The ratio of IgG1/IgG2a of the FI-RSV＋CpG group was significantly higher than that of FI-RSV＋ Al （OH）3 group. There are obvious signs of illness in FI-RSV＋ CpG group. Abundant mucus was seen in FI-RSV--Al（OH）3 group. After RSV attack, in the FI-RSV＋CpG group, there was infiltration of inflammatory cells, and the alveolar wall was obviously thickening, in addition, edema was found between alveolars. In FI-RSV＋AI（OH）a groups, the level of RSV-N expression was lower than that in the FLRSV＋CpG group. The mRNA expressions of IFN-γ, IL-5, TNF-α, GATA-3, T-bet in FI-RSV-Al（OH）3 group were higher than those of the FI-RSV＋Al（OH）3 group（P〈0.05）. The mRNA expressions of IL-17, RORyt in the FI-RSV＋CpG group were higher than those of the FI-RSV＋Al（OH）a group （P 〈 0.05）. Altogether, compared with Al（OH）3, CpG as adjuvants has no significant difference in the titers of IgG antibody and creates a strong inflammatory lung injury and pathology, whereas, Al（OH）3 as adjuvant leads to strong mucus secretion.

关 键 词：RSV 佐剂 疫苗 

分 类 号：R373.1[医药卫生—病原生物学]