碳源自供给潜流人工湿地中反硝化基因与碳氮比的关系  

Relationships Between Denitrifying Genes and C/N Ratio in Self-supplying Carbon Source Constructed Wetlands

作　　者：刘畅[1] 闻岳[1] 余雪岑 郭文瑞[1] 周琪[1] 

机构地区：[1]同济大学环境科学与工程学院,上海200092

出　　处：《山东化工》2017年第3期126-129,共4页Shandong Chemical Industry

基　　金：国家自然科学基金资助项目(51378372)

摘　　要：本研究构建了4套碳源自供给水平潜流人工湿地装置,以香蒲生物质发酵液作为外加碳源,考察了生物质发酵液投加量(进水碳氮比CODAdd/NO_3-N=1,2,3,4)与植物(Typha latifolia)对反硝化功能基因nirS、nirK和nosZ的影响。结果表明,三种基因在湿地系统中数量级分别为107~108、105和10~6~10~7拷贝数/g砾石。生物质发酵液能促进含nirS、nirK和nosZ的微生物生长;植物能促进含nirS、nirK微生物生长。In this study,four HSSF CWs were set up to investigated the effects of plant fermentation broth （ CODAdd/NO3-N = 1,2,3,4 ） and plants （Typha latifolia） on the denitrification genes. The results showed that the magnitude of copy numbers of the three genes in the wetland systems were 10^7 - 10^8 ,10^5 and 10^6 - 10^7 copies g^-1 The addition of fermentation broth could promote the growth of bacteria containing nirS,nirK and nosZ. Typha latifolia enhanced the growth of bacteria containing nirS and nirK.

关 键 词：水平潜流人工湿地 反硝化基因 生物质发酵液 碳源 脱氮 

分 类 号：X703.1[环境科学与工程—环境工程]