利用果胶降解菌塔宾曲霉GYC 501改善梗丝品质及其发酵条件的优化  

Application of Pectin-degrading Fungus Aspergillus tubingensis GYC 501 in Improvement of Tobacco Cut-stem Quality and Optimization of Its Fermentation Conditions

作　　者：龙章德[1] 陈皓睿 刘鸿[1] 邹克兴[1] 孙建生[1] 李季刚[1] 薛云[1] 申佩弘[2] 

机构地区：[1]广西中烟工业有限责任公司,广西南宁530001 [2]广西大学生命科学与技术学院,广西南宁530005

出　　处：《江西农业学报》2017年第2期95-98,共4页Acta Agriculturae Jiangxi

基　　金：广西科技厅科技攻关项目(桂科攻15118006)

摘　　要：烟草梗丝中含有大量果胶、纤维素等细胞壁物质,造成吸味不好,对其在烟制品中的应用造成了阻碍。本研究旨在利用GYC 501的发酵酶液处理烟草梗丝,以降解其中以果胶为主的多糖,改善吸味。本研究首先建立了一种测定烟草梗丝中果胶含量相对简易的方法,并从发酵时间、缓冲液浓度、缓冲液p H值、发酵温度、酶的使用量入手,先进行单因素优化实验再进行正交实验,以优化发酵条件。结果显示:使用塔宾曲霉(Aspergillus tubingensis)GYC 501在烟草梗丝培养基中发酵48 h后所得到的发酵液对烟草梗丝进行发酵处理,得到最优发酵条件:酶使用量为1536 U,发酵时间为12h,温度为42℃,缓冲液p H为4.8,缓冲液浓度为0.3 mol/L,梗丝含水量为50%。在此最优发酵条件下,梗丝果胶降解率最佳,为34.97%。There are a large amount of cell wall substances such as pectin and cellulose in tobacco stem,and these substances cause bad smoking quality,thus tobacco stem application is limited in manufacture. This research treated tobacco cut-stem with the fermentation liquid of Aspergillus tubingensis GYC 501 to degrade the polysaccharides（mainly being pectin） in it and to improve its smoking quality. A relative simple method for measuring the pectin content in tobacco cut-stem was built. The fermentation conditions（fermentation time,buffer solution concentration,buffer solution p H-value,fermentation temperature,pectinase application rate,and so on） of tobacco cut-stem were optimized by single-factor experiment and orthogonal test. The fermentation liquid of A. tubingensis GYC 501,which was obtained by culturing this fungus in the tobacco cut-stem liquid medium for 48 h,was applied to ferment the tobacco cut-stem. The optimal fermentation conditions were obtained as follows：pectinase application rate 1536 U,fermentation time 12 h,fermentation temperature 42 ℃,buffer solution p H 4.8,0.3 mol/L buffer solution,cut-stem moisture content 50%. Under the above optimum fermentation conditions,the degrading rate of pectin in tobacco cut-stem was the highest,reaching 34.97%.

关 键 词：烟草梗丝 塔宾曲霉 果胶酶 条件优化 正交试验 

分 类 号：Q815[生物学—生物工程]