CEA迷你基因串联体疫苗的体内抗肿瘤活性观察  

Observation of anti tumor activity in vivo of three tandem repeats of minigene DNA vaccine derived from CEA gene

作　　者：方艳秋[1] 魏海峰[1] 李丹[2] 米旭光 刘磊[1] 李首庆[1] 谭岩[1] FANG Yan-Qiu WEI Hai-Feng LI Dan MI Xu-Guang LIU Lei LI Shou-Qing TAN Yan(Center for Medical Treatment and Diagnosis , Jilin Province People＇s Hospital, Changchun 130021 ,China)

机构地区：[1]吉林省人民医院医学诊治实验中心,长春130021 [2]吉林大学第一医院呼吸内科,长春130012

出　　处：《中国免疫学杂志》2017年第1期62-65,共4页Chinese Journal of Immunology

基　　金：吉林省科技厅国际合作项目(20140414043GH);吉林省科技厅中青年科技创新领军人才及团队项目(20140519018JH);吉林省科技厅重点实验室项目(20122113)资助;吉林省人社厅省人才开发资金(2016年度)

摘　　要：目的:观察已构建的含有CEA_(625-667)基因单倍体疫苗pc DNA-CEA_(625-667)及三串联体的DNA疫苗pc DNA-triCEA_(625-667)对荷瘤小鼠体内肿瘤的抑制情况及小鼠存活时间的改变。方法:建立小鼠肝细胞癌实验动物模型,应用单倍体疫苗pc DNA-CEA_(625-667)及三串联体DNA疫苗pc DNA-tri CEA_(625-667)免疫小鼠,以生理盐水为对照组,观察各实验组小鼠皮下肿瘤生长情况,记录皮下肿瘤生长曲线,观察疫苗对荷瘤小鼠肿瘤生长速度的影响以及疫苗对荷瘤小鼠生存时间的影响。结果:与生理盐水对照组相比,两种疫苗均能明显抑制CEA阳性荷瘤小鼠的肿瘤体积以及生长速度(P<0.01),其中,pc DNA-tri CEA_(625-667)疫苗组的抑制作用明显优于pc DNA-CEA_(625-667)疫苗组(P<0.01),而二者均不能抑制CEA阴性荷瘤小鼠的肿瘤生长。pc DNACEA_(625-667)疫苗组平均生存时间为(48.50±6.73)d,与生理盐水对照组(39.00±6.64)d相比有显著差异(P<0.01);pc DNA-triCEA_(625-667)疫苗组生存时间(48.50±6.73)d明显高于生理盐水对照组和pc DNA-CEA_(625-667)疫苗组(P<0.01)。两组疫苗均不能延长CEA阴性荷瘤小鼠的生存时间。结论:无论是单倍体还是三串联体的DNA疫苗,均能够明显抑制CEA阳性荷瘤小鼠的肿瘤生长速度(P<0.01)及明显延长其生存时间(P<0.01),而对CEA阴性荷瘤小鼠则无治疗作用。Objective： To observe the inhibitory effect of haploid vaccine pc DNA-CEA_（625-667） and three tandem repeats of minigene DNA vaccine pc DNA-tri CEA_（625-667） derived from CEA gene on tumor in mice bearing tumor and the changes of survival time. Methods： The experimental animal model of mouse liver cell carcinoma was established and the mice were immunized with pc DNA-CEA_（625-667） and three series of DNA vaccine. Some of the mice were treated with normal saline as control group. The growth curve of tumor growth curve was recorded and the effect of vaccine on the survival time of tumor bearing mice was observed. Results： Compared with the normal saline control group,the two vaccines were able to significantly inhibit the tumor size and growth rate（ P〈0. 01） of CEA positive tumor bearing mice,the inhibition of pc DNA-tri CEA_（625-667） vaccine group was significantly better than the pc DNA-CEA_（625-667） vaccine group（ P〈0. 01）,while the two were not inhibited tumor growth in CEA negative tumor bearing mice. The average survival time of the pc DNA-CEA_（625-667） vaccine group was（ 48. 50 ± 6. 73） d,and there was significant difference（ P〈0. 01） compared with the saline control group（ 39. 00 ± 6. 64） d. The survival time（ 48. 50 ± 6. 73） d of the pc DNA-tri CEA_（625-667） vaccine group was significantly higher than that of the normal saline control group and the pc DNA-CEA_（625-667） vaccine group（ P〈0. 01）. The survival time of CEA negative tumor bearing mice could not be prolonged in the two groups. Conclusion： Either the haploid or the three series of the DNA vaccine,were able to significantly inhibit tumor growth rate（ P〈0. 01） and significantly prolong the survival time（ P〈0. 01） of CEA positive tumor bearing mice,but they had no therapeutic effect on CEA negative tumor bearing mice.

关 键 词：癌胚抗原 迷你基因 基因疫苗 体内实验 抗肿瘤 

分 类 号：R392[医药卫生—免疫学] R730.5[医药卫生—基础医学]